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用重复序列探针MGR586与限制性内切酶EcoR1组合,分析了11个稻瘟病菌(无性/有性)菌丝自身融合的亲代菌株及其22个菌丝融合点的再度培养菌株,以及7对相互组配后的第5代菌丝融合菌株的限制性片段长度多态性(RFLPs),依其MGR-DNA指纹的相似性,其中大多数菌株被归属于不同的系谱;结合病菌的致病性测定,亦可鉴别出许多互不相同的生理小种。初步证实了稻瘟病菌本身存在的菌丝融合现象是该菌生理小种致病性变异的原因之一。
Using the repeat sequence probe MGR586 in combination with the restriction endonuclease EcoR1, the parental strain of 11 M. grisea (asexual / sexual) mycelia autonomously fused and their 22 mycelial fusion point re-cultured strains, as well as 7 Restriction fragment length polymorphisms (RFLPs) of the fifth-generation mycelium fusion strains after mating with each other, most of which belong to different pedigree according to their MGR-DNA fingerprinting; Pathogenesis, but also identify many different races. The preliminary confirmation of mycelial fusion existing in M. grisea itself is one of the reasons for the pathogenicity variation of the physiological races of this strain.