前列腺素 F_2α(PGF_2α)促进牝马的黄体消退,但机理不明,为探讨 PGF_2α的作用方式,可采用放免法(RIA)测定孕酮和白三烯 B-4(LTB-4)观察 PGF_2α在体外对牝马间情期不同阶段黄体细胞分泌孕酮和 LTB-4的影响。标准饲养3～12年的牝马(n=7) ,在动情期开始每天用超声经直肠探测卵巢以确定排卵日期。分别在排卵后第4～5天(间情早期,n=4) Prostaglandin F_2α (PGF_2α) can promote the luteal regression of equine, but its mechanism is not clear. To explore the mode of action of PGF_2α, PGF 2α can be detected by RIA in vitro Effects of Progesterone and LTB-4 Secretion by Luteinizing Cells in Different Stages of Emu Emulsion. Standard feeding 3 to 12 years of gelding (n = 7), in the estrus began every day using ultrasound to detect the ovary rectum to determine the date of ovulation. Respectively after ovulation 4 to 5 days (early estrus, n = 4)