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目的探讨HMGB1/RAGE蛋白在食管鳞癌中的表达及其对食管鳞癌预后判定的作用。方法以食管鳞癌旁正常组织40例为对照,免疫组化检测80例食管鳞癌组织HMGB1、RAGE蛋白的表达,分析HMGB1、RAGE表达与临床病理因素的关系及其对食管鳞癌预后的影响。结果HMGB1、RAGE蛋白在食管鳞癌组织和正常鳞状上皮的阳性表达率分别为47.5%vs17.5%和72.5%vs42.5%,差异均具有统计学意义;HMGB1、RAGE蛋白的阳性表达与食管鳞癌浸润深度、淋巴结转移及TNM分期正相关(P<0.05),与癌细胞分化程度负相关(r分别为-0.51、-0.242,P<0.05);HMGB1和RAGE之间的表达呈正相关(r=0.35,P=0.001);HMGB1、RAGE蛋白阳性表达食管鳞癌患者预后较差,COX分析显示HMGB1蛋白为影响食管鳞癌预后的独立因素(HR=4.853,P=0.000)。结论HMGB1和RAGE蛋白的表达与食管鳞癌的临床病理学特征密切相关,HMGB1与其受体RAGE的结合可能参与了食管鳞癌的侵袭和转移,HMGB1可作为预测食管鳞癌预后的重要指标。
Objective To investigate the expression of HMGB1 / RAGE protein in esophageal squamous cell carcinoma and its effect on the prognosis of esophageal squamous cell carcinoma. Methods 40 cases of normal esophageal squamous cell carcinoma tissue as control, immunohistochemical detection of 80 cases of esophageal squamous cell carcinoma HMGB1, RAGE protein expression, analysis HMGB1, RAGE expression and clinicopathological factors and prognosis of esophageal squamous cell carcinoma . Results The positive expression rates of HMGB1 and RAGE protein in esophageal squamous cell carcinoma and normal squamous epithelium were 47.5% vs 17.5% and 72.5% vs 42.5%, respectively, with statistical significance; the positive expressions of HMGB1 and RAGE protein were There was a positive correlation between HMGB1 and RAGE (P <0.05), but negatively correlated with the degree of differentiation (r = -0.51, -0.242, P <0.05, respectively) (r = 0.35, P = 0.001). The prognosis of esophageal squamous cell carcinoma patients with HMGB1 and RAGE protein expression was poor. COX analysis showed that HMGB1 protein was an independent factor affecting the prognosis of esophageal squamous cell carcinoma (HR = 4.853, P = 0.000). Conclusion The expressions of HMGB1 and RAGE are closely related to the clinicopathological features of esophageal squamous cell carcinoma. The combination of HMGB1 and its receptor RAGE may be involved in the invasion and metastasis of esophageal squamous cell carcinoma. HMGB1 may be used as an important index to predict the prognosis of esophageal squamous cell carcinoma.