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针对白术的不同加工方法,观察白术在硫磺熏蒸、不同温度鼓风烘制和微波灭菌的加工方式下,样品对脾虚大鼠的唾液淀粉酶及尿中D-木糖排泄率的影响。大鼠分为空白对照组,大黄所致脾虚模型对照组和不同加工方式的白术实验组,分别采用淀粉酶比色测量法和邻苯甲胺法测定大鼠唾液淀粉酶活性和尿D-木糖排泄率。与空白对照组比较,发现100,110℃鼓风烘制的白术均可增加大鼠唾液淀粉酶的单位含量,脾虚大鼠的唾液淀粉酶升高,同时可拮抗大鼠尿中D-木糖排泄率降低的现象,与模型组比较有较明显差异(P<0.05);与模型组相比,70℃烘制及微波处理的白术相对于其他药液作用较强,较模型组有显著性差异(P<0.01)。白术能改善脾虚大鼠对D-木糖吸收功能和唾液淀粉酶活性,且尿中D-木糖排泄率的改变与唾液淀粉酶活性呈正相关。不同温度鼓风烘制和微波灭菌加工的白术对脾虚大鼠唾液淀粉酶活性和尿中D-木糖排泄率的影响较小,而硫磺熏蒸对脾虚大鼠唾液淀粉酶活性和尿中D-木糖排泄率的影响较大。
Atractylodes macrocephala was treated with different processing methods to observe the effect of Atractylodes macrocephala on the salivary amylase and urine D-xylose excretion rate of rats with spleen deficiency syndrome under sulfur fumigation, air blast drying and microwave sterilization. Rats were divided into blank control group, rhubarb spleen deficiency model control group and different processing methods of Atractylodes macrocephalus experimental group, respectively, by amylase colorimetric assay and o-benzylamine method for the determination of rat saliva amylase activity and urinary D-wood Sugar excretion rate. Compared with the blank control group, it was found that the atractylodes macrocephalae treated with air blowing at 100 and 110 ℃ increased the unit content of salivary amylase in rats, the salivary amylase in spleen deficiency rats and the excretion rate of D-xylose in rat urine (P <0.05). Compared with the model group, Atractylodes macrocephala that was baked at 70 ℃ and microwave treatment had a stronger effect than the other solutions, which was significantly different from the model group (P < P <0.01). Atractylodes macrocephalae can improve the absorption of D-xylose and salivary amylase activity in spleen-deficient rats, and the change of urinary excretion rate of D-xylose is positively correlated with salivary amylase activity. Aphrodisiac treatment at different temperatures and microwave sterilization process of saliva amylase activity in spleen and urinary excretion of D-xylose little effect, and sulfur fumigation of spleen deficiency saliva amylase activity and urinary D - Xylose excretion rate greater impact.