不同抑制物质处理对杂交水稻种子活力和胚贮藏蛋白的影响

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本文研究了穗萌抑制剂 (IS)、比久 (B9)、多效唑 (PP333)三种抑制物质田间处理对杂交水稻组合协优 46 (X46 )和汕优 10号 (S10 )种子生活力和胚贮藏蛋白的影响。结果表明 ,抑制物质处理有利于保持杂交水稻种子的生活力和活力 ,其中以 IS处理效果最为明显 ,经常温贮藏 2 0个月后 ,IS处理的 X46和 S10种子发芽率分别比对照高 19.0 %和 12 .5 % ,发芽指数分别比对照提高 6 .7和 6 .5。不同生活力的杂交水稻种子 ,吸胀 2 4小时后的胚贮藏蛋白电泳图谱有显著差异 ,在分子量为 45 .0~ 2 0 5 .0 KD之间最为明显 ,生活力和活力较高的种子 ,其胚贮藏蛋白谱带条数和浓度皆多于或高于低生活力和低活力种子。 In this paper, we studied the effects of spike germination inhibitor (IS), Bijiaobu (B9) and paclobutrazol (PP333) on soil seed viability and embryo development of hybrid rice combinations Xiyou 46 (X46) and Shanyou 10 Effect of storage proteins. The results showed that the inhibitory substance treatment was beneficial to maintain the viability and viability of hybrid rice seeds. Among them, IS treatment had the most obvious effect. After 20 months of storage at normal temperature, the germination percentage of X46 and S10 seeds treated with IS were 19.0% And 12.5% ​​respectively, the germination index increased by 6.7 and 6.5 respectively compared with the control. The hybrid rice seeds with different viability had significant differences in the electrophoretogram of embryo storage proteins after 24 hours of imbibition, with the most obvious difference in the molecular weights of 45.0-2.05.0 KD, with higher viability and vigor , The embryo storage protein band number and concentration are more or less than the low viability and low activity seeds.
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