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本实验利用大鼠甩尾测痛结合脊髓蛛网膜下腔注药的方法，观察了脊髓水平内源性阿片样物质（ＯＬＳ）对５一羟色胺（５—ＨＴ）４痛作用的影响。实验结果显示；（１）脊髓蛛网膜下腔注射６０ｎｍｏｌ的５—ＨＴ可产生明显的镇痛作用，并可为同一途径注射阿片受体阻断剂纳络酮所完全阻断；（２）同一剂量的纳络酮也能阻断鞘内注射６０ｎｍｏｌ吗啡所引起的镇痛作用；（３）单独使用１２０ｎｍｏｌ纳络酮对痛阈无明显影响。以上结果表明．在脊髓水平５—ＨＬ的镇痛作用是通过ＯＬＳ介导的。
In this study, we investigated the effect of endogenous spinal opioid (OLS) on 5-hydroxytryptamine (5-HT) 4 pain by using the method of tail flicking and painful spinal subarachnoid injection in rats. The experimental results showed that: (1) Spinal subarachnoid injection of 60nmol of 5-HT can produce significant analgesic effect, and can be completely blocked by the same route of injection of opioid receptor blocker naloxone; (2) the same The dose of naloxone also blocked intrathecal injection of 60nmol morphine induced analgesic effect; (3) 120nmol naloxone alone had no significant effect on the pain threshold. The above result shows. The analgesic effect of 5-HL at spinal level is mediated by OLS.