The mixed quark-gluon condensate from the global color symmetry model is derived. It is shown that themixed quark-gluon condensate depends explicitly on the gluon propagator. This interesting feature may be regarded asan additional constraint on the model of gluon propagator. The values of the mixed quark-gluon condensate from someansatz for the gluon propagator are compared with those determined from QCD sum rules.