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目的:建立不同采收期青龙衣药材HPLC指纹图谱。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(250×4.6mm,5μm);流动相为甲醇-1%冰醋酸溶液进行梯度洗脱,检测波长为270nm;流速1.0 mL/min;柱温为30℃。结果:7~8月样品指纹图谱整体相似度高于9~10月,以7~8月采收为佳。结论:该方简便可行,重复性和精密度良好,为全面控制青龙衣药材的质量提供可靠的科学依据。
Objective: To establish HPLC fingerprints of Qinglongyi medicinal materials in different harvest time. Methods: The mobile phase consisted of Diamonsil C18 (250 × 4.6 mm, 5 μm) with gradient elution with methanol-1% glacial acetic acid. The detection wavelength was set at 270 nm. The flow rate was 1.0 mL / min. The temperature is 30 ° C. Results: The overall similarity of fingerprints of samples from July to August was higher than that of September to October, and it was better to harvest from July to August. Conclusion: The prescription is simple and feasible, reproducibility and precision are good, which provide a reliable scientific basis for the overall control of the quality of Qinglongyi medicinal materials.