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目的:探讨NF-κB、IL-17在儿童支气管哮喘中的作用及机制。本文通过观察哮喘急性发作儿童支气管肺泡灌洗液(BALF)NF-κB、IL-17的水平变化,探讨其在哮喘急性发作儿童气道炎症中的作用。方法:我院2012年12月-2013年2月期间行纤维支气管镜检查患儿共80例,包括哮喘急性发作组(哮喘组,n=40)、非喘息组(肺炎组,n=20)及对照组(n=20),收集所有病例的BALF,进行细胞学分类,RT-PCR法测定BALF中细胞中NF-κB蛋白(P65mRNA)、IL-17mRNA的表达;Western法检测P65蛋白、IL-17蛋白表达。结果:与对照组比较,哮喘组和肺炎组患儿的P65mRNA、IL-17mRNA水平均明显增高;P65蛋白、IL-17蛋白水平均明显增高(均P<0.05);哮喘组患儿的P65mRNA、IL-17mRNA水平、P65蛋白、IL-17蛋白水平较肺炎组高(P<0.05)。结论:NF-κB、IL-17在哮喘儿童气道炎症中发挥重要作用,NF-κB通过调控IL-17来实现促进哮喘病情进展。
Objective: To investigate the role and mechanism of NF-κB and IL-17 in children with bronchial asthma. In this paper, the changes of NF-κB and IL-17 in bronchoalveolar lavage fluid (BALF) in children with acute asthma were observed to explore their roles in airway inflammation in children with acute asthma. Methods: A total of 80 children underwent fibrobronchoscopy in our hospital from December 2012 to February 2013, including acute asthma attack group (n = 40 in asthma group), non-wheezing group (pneumonia group, n = 20) And control group (n = 20). BALF was collected in all cases for cytological classification. The expression of NF-κB protein and IL-17 mRNA in BALF was detected by RT-PCR. -17 protein expression. Results: Compared with the control group, the levels of P65 mRNA and IL-17 mRNA in asthma group and pneumonia group were significantly increased, the levels of P65 protein and IL-17 protein were significantly increased (all P <0.05); P65 mRNA, The levels of IL-17 mRNA, P65 protein and IL-17 protein were higher than those of pneumonia group (P <0.05). Conclusion: NF-κB and IL-17 play an important role in airway inflammation in children with asthma. NF-κB can promote the progress of asthma by regulating IL-17.