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目的建立保肾胶囊含量测定方法。方法以大黄和元明粉为原料混合制成保肾胶囊,大黄素为对照品,采用高效液相色谱法测定含量,选用HypersilODS色谱柱(150mm×4.6mm,4.5μm),流动相为甲醇-0.1%磷酸溶液,检测波长254nm。结果测定结果显示在2.4～28.8μg.mL-1浓度范围内吸光度与浓度呈线性关系,r=0.9999。加样回收率在98.7～102.8%之间,平均回收率为100.8%,标准偏差为1.25%。结论测定结果为临床合理用药提供科学依据。
Objective To establish a method for the determination of Baoshen Capsule. Methods Rhubarb and Yuanming Ming as raw materials were mixed into Baoshen Capsule. Emodin was used as reference substance, and the content was determined by high performance liquid chromatography. Hypersil ODS column (150mm × 4.6mm, 4.5μm) was used with the mobile phase of methanol - 0.1% phosphoric acid solution, detection wavelength 254nm. The results showed that the absorbance in the concentration range of 2.4 ~ 28.8μg.mL-1 showed a linear relationship with the concentration, r = 0.9999. The recoveries of samples ranged from 98.7% to 102.8% with an average recovery of 100.8% and a standard deviation of 1.25%. Conclusion The results provide a scientific basis for clinical rational use of drugs.