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建立头孢克罗在人血浆及尿中的ＨＰＬＣ快速测定方法，选用分析柱为Ｈｙｐｅｒｓｉｌ Ｃ１８（４．６ｘ２００ｍｍ，５ｍ），流动相采用甲醇：四氢呋喃：水（１Ｌ水中含庚烷磺酸钠０．５ｇ、三乙胺７．５ｍｌ。磷酸６ｍｌ，ｐＨ２．５）为２３∶４∶７３，流速１．０ｍｌ／ｍｉｎ；检测波长２６５ｎｍ。选择８名男性健康志愿者单剂量口服５００ｍｇ，应用所建方法研究其在健康人体内的药代动力学。研究结果表明：血药浓度在０．１０～２５．０ｇ／ｍｌ范围内线性良好（＝０．９９９９），尿药浓度在１～２５０ｇ／ｍｌ范围内线性良好（＝０．９９９９），方法的日内及日间精密度均小于１０％，回收率也符合体内药物分析的要求。测得希刻劳胶囊的Ｃｍａｘ为１３．１７±４．７６ｇ／ｍｌ；Ｔｍａｘ为０．６２±０．２０ｈ；ｔ１／２为０．６３±０．２０ｈ；ＡＵＣ０为１８．３０±３．６２ｈ／（ｇ·ｍｌ）。
The HPLC method for determination of cefaclor in human plasma and urine was established. The analytical column was Hypersil C18 (4.6 × 200 mm, 5m). The mobile phase consisted of methanol: tetrahydrofuran: water (0.5g heptane sulfonate in 1L water) , Triethylamine 7.5ml. Phosphoric acid 6ml, pH2.5) 23:4:73, flow rate 1.0ml / min; detection wavelength 265nm. Eight healthy male volunteers were selected to take 500 mg orally. The pharmacokinetics of the drug in healthy volunteers were studied using the established method. The results showed that the linearity was good (= 0.9999) in the range of 0.10-25.0 g / ml, the linearity was good in the range of 1-250 g / ml (= 0.9999) Intra-day and intra-day precision were less than 10%, the recovery rate also in line with the requirements of in vivo drug analysis. The measured Cmax of Xijiaole capsule was 13.17 ± 4.76g / ml; Tmax was 0.62 ± 0.20h; t1 / 2 was 0.63 ± 0.20h; AUC0 was 18.30 ± 3.62h / (G · ml).