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目的对比分析乙型肝炎纤维化和非纤维化肝组织的蛋白质组表达谱的差异,探讨二甲基精氨酸二甲基氨基水解酶1(DDAH1)在肝纤维化发生发展中的功能,乙型肝炎病毒抗DDAH1与抗HBc-IgM检测的差异及其临床意义。方法 360例血清标本同时测定抗HBc-IgG与抗HBc-IgM,前者是按常用竞争法,后者用捕捉法进行。结果抗HBc-IgM的检测在急性乙型肝炎的诊断中较抗HBc-IgG的检测更有价值。结论乙型肝炎肝纤维化与非纤维化肝组织的蛋白质表达谱有一定差异,DDAH1有可能通过调节ADMA的代谢而影响NO合成,参与了肝纤维化的发生发展。
OBJECTIVE: To compare the difference of proteome expression profiles between hepatitis B fibrosis and non-fibrosis liver tissues and to explore the function of dimethylarginine dimethylaminohydrolase 1 (DDAH1) in the development and progression of hepatic fibrosis. Difference of Hepatitis A Virus Detecting Anti-DDAH1 and Anti-HBc-IgM and Its Clinical Significance. Methods 360 serum samples were simultaneously tested for anti-HBc-IgG and anti-HBc-IgM, the former by the commonly used competition method, the latter by the capture method. Results The detection of anti-HBc-IgM was more valuable than the anti-HBc-IgG in the diagnosis of acute hepatitis B Conclusion The protein expression profiles of liver fibrosis and non-fibrotic liver tissue of hepatitis B are different. DDAH1 may affect the NO synthesis by regulating the metabolism of ADMA, and is involved in the development of liver fibrosis.