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本课题受国家重点基础研究发展规划基础基金项目(G1998051211)和上海市医学领先专业基金项目(983001)资助,围绕肝癌转移复发这一重大问题,建立了高转移人肝癌裸小鼠模型和细胞系,并进行了转移机制及干预研究。 1.首先采用原位移植策略,将肝癌病人的肝内转移灶接种到雄性裸小鼠肝内传代并筛选,建成高转移人肝癌裸鼠模型LCI-D20,该模型模拟了肝癌病人的临床病理过程。潜伏期1周,第2周在呈肝
This topic was supported by the National Basic Research Foundation of China (G1998051211) and the Shanghai Medical Leading Professional Fund Project (983001). On the major issue of metastasis and recurrence of liver cancer, a model of high metastatic human hepatocellular carcinoma in nude mice and a cell line , And conducted a transfer mechanism and intervention research. First, the orthotopic transplantation strategy was used to inoculate the intrahepatic metastases of liver cancer patients into the liver of male nude mice for passage and screening to establish LCI-D20 model of high metastatic human hepatocellular carcinoma in nude mice. The model simulates the clinicopathological features of patients with hepatocellular carcinoma process. Latency 1 week, 2 weeks in the liver