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目的:观察参附注射液(Shenfu injection,SF)对血栓闭塞性脉管炎(thromboangitis obliterans,TAO)模型大鼠的防治作用及其机制。方法:健康成年雄性SD大鼠,随机分为5组,即假手术组、TAO模型组、SF低、中、高剂量(2.5,5,10 mg.kg-1)组。采用月桂酸股动脉注射制作大鼠TAO模型。经尾静脉给予生理盐水或低、中和高剂量SF,连续15 d。观察大鼠的体征变化、病理切片血栓分级、测定血小板数量、血浆血栓素B2(thromboxane B2,TXB2)、6-酮基-前列腺素F1α(6-keto-prostaglandin F1α,6-K-PGF1α)含量等指标。结果:SF明显抑制血管内血栓形成;SF中,高剂量组血小板计数较模型组显著减少(分别为P<0.05和P<0.01)。另外,SF中、高剂量组血浆TXB2也明显减少(P<0.01)而6-K-PGF1α显著增加(P<0.01)。结论:SF可能通过其加强对血小板聚集的抑制作用,提高血管的抗血栓功能,减少TAO模型大鼠血栓形成,以及改善其病变体征。
Objective: To observe the preventive and therapeutic effects of Shenfu injection (SF) on rat model of thromboangitis obliterans (TAO) and its mechanism. Methods: Healthy adult male Sprague-Dawley rats were randomly divided into 5 groups: sham operation group, TAO model group, SF low, medium and high dose (2.5, 5, 10 mg.kg-1) groups. Rat LAO model was made by injection of lauric acid femoral artery. Saline was administered via tail vein or low, medium and high dose SF for 15 days. The changes of the signs of the rats were observed, the thrombosis grades of pathological sections, the number of platelets, the contents of thromboxane B2 (TXB2) and 6-Keto-prostaglandin F1α And other indicators. Results: SF significantly inhibited intravascular thrombosis. The platelet count of medium and high dose SF group was significantly lower than that of model group (P <0.05 and P <0.01, respectively). In addition, the levels of TXB2 in plasma were significantly decreased (P <0.01) and 6-K-PGF1α significantly increased (P <0.01). Conclusion: SF may enhance the inhibition of platelet aggregation, increase the antithrombotic function of blood vessels, reduce the thrombosis in TAO model rats, and improve the signs of the disease.