用鼠细胞融合杂交瘤技术,获得3株分泌抗人T淋巴细胞表面抗原的单克隆抗体杂交瘤细胞株,分别为SMU_1、SMU_2、SMU_3,为IgG_1类,不与补体结合。间接免疫荧光法表明,SMU_1、SMU_3分别与50.3±6.42%和51.5±6.37%的周血单个核细胞反应,SMU_2与68.89±9.92%的周血半个核细胞及74.68±8.08%的成熟粒细胞反应,3株单抗的分子量为54～62kD(未还原)。调变试验及竞争抑制试验表明SMU_1、SMU_3与WuT_3识别相同的抗原分子,但为不同的表位。SMU_2为一非系限性单抗。本组单抗对研究T淋巴细胞分化及临床白血病免疫分型是有意义的。 Three murine monoclonal antibody hybridoma cell lines secreting anti-human T lymphocyte surface antigen were obtained by using murine cell fusion hybridoma technology. They were SMU_1, SMU_2 and SMU_3, which were IgG_1 class, and did not bind with complement. Indirect immunofluorescence assay showed that SMU_1 and SMU_3 reacted with 50.3 ± 6.42% and 51.5 ± 6.37% of peripheral blood mononuclear cells, SMU_2 with 68.89 ± 9.92% of peripheral blood hemocytes and 74.68 ± 8.08% of mature myocytes, respectively The molecular weights of the three mAbs ranged from 54 to 62 kD (unreduced). Modulation tests and competition inhibition tests showed that SMU_1, SMU_3 and WuT_3 recognize the same antigen molecules but different epitopes. SMU_2 is a non-limiting monoclonal antibody. This group of monoclonal antibodies for the study of T lymphocyte differentiation and clinical leukemia immunophenotyping is meaningful.