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前期研究发现,重要的外来入侵害虫B型烟粉虱Bemisia tabaci在烟草植株上对烟蚜Myzus persicae具有明显的种间竞争优势。为明确两种害虫的生理适应性差异对其种间竞争的影响,用生化分析法研究了两种成虫取食B型烟粉虱为害的烟草植株后其体内主要保护酶和消化酶活性的影响。结果表明:B型烟粉虱取食其前侵染的烟草植株6,12,24,48和72h后,其体内超氧化物歧化酶(SOD)和多酚氧化酶(PPO)的活性均显著增加(P<0.05),过氧化物酶(POD)活性在取食12和24h后变化不明显,但在取食6,48和72h后也显著增加(P<0.05),B型烟粉虱体内的这3种酶活性的最高值较对照最高分别增加达1.62,2.71和2.57倍。烟蚜取食B型烟粉虱前侵染的烟草植株在多数取食时间段内,其体内PPO和SOD的活性仅略有增加,但在取食12h后SOD的活性受到明显的抑制。POD活性除在取食24h后显著高于对照外,在取食其他时间后均受到显著的抑制(P<0.05)。B型烟粉虱取食其前侵染的烟草植株不同时间后,其体内蛋白酶和淀粉酶活性均显著增加,最大值分别是对照的1.54倍和1.33倍;烟蚜取食烟草植株6h后,其体内蛋白酶和淀粉酶活性与对照相比没有明显差异(P<0.05),但随着取食时间的延长,这两种酶活性被显著抑制,其最低值分别为对照的0.39和0.72倍。结果提示:与烟蚜相比,B型烟粉虱取食经B型烟粉虱前侵染的烟草后其体内保护酶和消化酶的活性提高是其具有较高适应性的原因之一。
Previous studies have found that Bemisia tabaci, an important invasive pest insect, has significant interspecies competition in the tobacco plant Myzus persicae. In order to clarify the effect of differences in physiological adaptability between the two pests on their interspecific competition, the biochemical analysis was used to study the effects of two adults on the activities of major protective enzymes and digestive enzymes in tobacco plants infected with B. tabaci. . The results showed that the activity of superoxide dismutase (SOD) and polyphenol oxidase (PPO) in B. tabaci B was significantly increased after 6, 12, 24, 48 and 72 h (P <0.05). The activity of peroxidase (POD) did not change obviously after 12 and 24 h of feeding, but also significantly increased after 6, 48 and 72 h of feeding (P <0.05) In vivo, the highest values of these three enzyme activities increased by 1.62, 2.71 and 2.57 times, respectively, as compared with the highest in the control. Tobacco plants infected with B.tabaci B before feeding on B. tabaci had only a slight increase in PPO and SOD activities during most of their feeding time periods, but SOD activity was significantly inhibited after 12 h of feeding. POD activity was significantly inhibited (P <0.05) except for 24 h after inoculation, but significantly after feeding at other times. B-type B. tabaci fed on tobacco plants that had previously been infected had significantly increased protease and amylase activities at 1.54-fold and 1.33-fold, respectively, in the tobacco plants infected with B. tabaci. The tobacco aphids fed on tobacco plants for 6 hours, The protease and amylase activities in vivo were not significantly different from those in the control (P <0.05). However, with the prolongation of feeding time, the activities of protease and amylase were significantly inhibited, the lowest values were 0.39 and 0.72 times of the control respectively. The results suggested that compared with the control, the B-type B. tabaci fed on the tobacco plants infected with B. tabaci B upgrowth increased the activity of protective enzymes and digestive enzymes, which was one of the reasons for its higher adaptability.