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目的:探讨去氧鬼臼毒素（DPPT）是否通过调控长链非编码RNA ZFPM2-AS1（LncRNA ZFPM2-AS1）/微小RNA-515-5p（miR-515-5p）分子轴而抑制结肠癌细胞增殖、迁移及侵袭。方法:体外培养人结肠癌LoVo细胞，随机分为Control组、DPPT-L组、DPPT-M组、DPPT-H组。甲基噻唑基四唑（MTT）检测细胞增殖；Transwell小室实验检测迁移及侵袭；实时荧光定量聚合酶链反应（qRT-PCR）检测ZFPM2-AS1、miR-515-5p的表达水平；LoVo细胞中转染pcDNA-ZFPM2-AS1，采用上述方法检测细胞增殖、迁移及侵袭；双荧光素酶报告实验验证ZFPM2-AS1、miR-515-5p的靶向关系；蛋白免疫印迹法（Western blot）检测细胞周期蛋白1（CyclinD1）、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）、P21的表达量。结果:与Control组比较，DPPT-L组、DPPT-M组、DPPT-H组细胞存活率降低（n P<0.05），迁移细胞数、侵袭细胞数减少（n P<0.05），CyclinD1、MMP-2、MMP-9蛋白水平降低（n P<0.05），P21蛋白水平升高（n P<0.05），ZFPM2-AS1表达水平降低（n P<0.05），miR-515-5p表达水平升高（n P<0.05），且DPPT-L组、DPPT-M组、DPPT-H组上述指标比较，差异均有统计学意义（n P<0.05）；ZFPM2-AS1可靶向结合miR-515-5p；与DPPT-H+pcDNA组比较，DPPT-H+pcDNA-ZFPM2-AS1组细胞存活率升高（n P<0.05），迁移细胞数、侵袭细胞数增多（n P<0.05），CyclinD1、MMP-2、MMP-9蛋白水平升高（n P<0.05），P21蛋白水平降低（n P<0.05）。n 结论:DPPT可能通过下调ZFPM2-AS1的表达及上调miR-515-5p的表达从而抑制结肠癌细胞增殖、迁移及侵袭。“,”Objective:To investigate whether deoxypodophyllotoxin (DPPT) inhibits proliferation, migration and invasion of colon cancer cells by regulating the long non-coding RNA ZFPM2-AS1/microRNA-515-5p (miR-515-5p) molecular axis.Methods:Human colon cancer LoVo cells were cultured in vitro and randomly divided into the control group, DPPT-L group, DPPT-M group, and DPPT-H group. Methylthiazolyl tetrazole (MTT) assay was used to assess cell proliferation; Transwell chamber assay was used to detect cell migration and invasion; real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression levels of ZFPM2-AS1 and miR-515-5p. Using these tests, LoVo cells were transfected with pcDNA-ZFPM2-AS1, and examined for proliferation, migration and invasion. Dual luciferase reporter assay was used to determine the targeting relationship between ZFPM2-AS1 and miR-515-5p. Western blotting was used to detect the expression of Cyclin 1, matrix metalloproteinase (MMP) -2, MMP-9, and P21 in the cells.Results:Compared with the control group, the DPPT-L, DPPT-M, and DPPT-H groups showed decreased cell viability (n P<0.05) , fewer migrating cells and invasive cells (n P<0.05) , lower expression levels of CyclinD1, MMP-2, and MMP-9 proteins (n P<0.05) , higher P21 protein level (n P<0.05) , lower ZFPM2-AS1 expression (n P<0.05) , and higher miR-515-5p expression (n P<0.05) . These measurements did not differ significant among DPPT-L, DPPT-M and DPPT-H groups (n P<0.05) . ZFPM2-AS1 was shown to target miR-515-5p. Compared with the DPPT-H+pcDNA group, the DPPT-H+pcDNA-ZFPM2-AS1 group showed increased cell viability (n P<0.05) , more migrating cells and invasive cells (n P<0.05) , higher protein expression of CyclinD1, MMP-2 and MMP-9 (n P<0.05) , and lower protein expression of P21 (n P<0.05) .n Conclusion:DPPT may inhibit the proliferation, migration and invasion of colon cancer cells by down-regulating the expression of ZFPM2-AS1 and up-regulating the expression of miR-515-5p.