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以泡桐花粉为受体,在超声波的作用下将含有绿色荧光蛋白(GFP)基因的质粒与花粉共处理,利用荧光显微镜对GFP基因在花粉粒及花粉管中的表达进行了示踪观察。结果表明:100g/L的蔗糖溶液是泡桐花粉较适宜的体外培养液;花粉粒有较强烈的自发荧光,因此不能根据花粉粒荧光来确定GFP基因是否表达;处理组花粉管较对照呈现强烈的绿色荧光,表明GFP基因在花粉管中表达。该试验首次利用泡桐花粉对GFP基因的表达观察,初步证明了这一转基因方法对泡桐花粉转化的可行性。
Using the Paulownia pollen as receptor, the plasmid containing green fluorescent protein (GFP) gene was co-treated with pollen under the action of ultrasound, and the expression of GFP gene in pollen grains and pollen tube was observed by fluorescence microscopy. The results showed that 100g / L sucrose solution was the more suitable in vitro culture medium for P. paulowniae pollen; pollen grains had stronger autofluorescence, so GFP gene expression could not be determined based on the fluorescence of pollen grains; pollen tube in treatment group showed stronger than the control Green fluorescence indicates that the GFP gene is expressed in the pollen tube. The experiment for the first time using the Paulownia pollen GFP gene expression was observed, preliminary proof of the transgenic method of Paulownia pollen transformation feasibility.