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目的探索单精子卵胞浆内注射(ICSI)前最适合的卵母细胞体外培养时间,即取卵结束到开始ICSI的时间(t),以利于取得高的受精率、优质胚胎率和良好的妊娠结局。方法根据卵母细胞体外培养时间,将患者分为3组:A组为2 h0.05)。(2)C组受精率(88.3%)显著高于A组和B组,差异有统计学意义(P<0.05)。C组优质胚胎率(41.6%)、植入率(18.7%)、妊娠率(30.7%)均低于A组和B组,差异有统计学意义(P<0.05)。C组卵裂率(98.9%)、流产率(12.0%)与A组和B组比较差异均无统计学意义(P>0.05)。结论过长卵母细胞体外培养时间(P>6 h)可以有高的受精率,但优质胚胎率降低,不利于改善ICSI患者的临床结局;卵母细胞体外培养2~6h后进行ICSI可以获得较为理想的临床结局。
Objective To explore the most suitable time for in vitro culture of oocytes prior to intracytoplasmic sperm injection (ICSI), that is, the time from the end of ovulation to the beginning of ICSI (t) in order to obtain high fertilization rate, good embryo rate and good Pregnancy outcome. Methods According to the oocyte culture time in vitro, the patients were divided into 3 groups: group A was 2 h 0.05). (2) The fertilization rate in group C (88.3%) was significantly higher than that in group A and group B (P <0.05). The quality embryo rate (41.6%), implantation rate (18.7%) and pregnancy rate (30.7%) in group C were significantly lower than those in group A and B (P <0.05). The cleavage rate (98.9%) and abortion rate (12.0%) in group C were not significantly different from those in group A and group B (P> 0.05). Conclusion Oocyte oocyte culture in vitro (P> 6 h) can have a high fertilization rate, but the rate of high quality embryos decreases, which is not conducive to improving the clinical outcome in patients with ICSI. Oocytes cultured in vitro for 2 ~ 6 h can be obtained after ICSI The ideal clinical outcome.