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[目的]探讨生命早期西玛津染毒对仔鼠的生殖毒性。[方法]成年SD大鼠雌性90只,雄性45只,按照2∶1进行合笼。选取状态良好的孕鼠80只,随机分为8组,在孕期和孕乳期通过灌胃染毒西玛津,分别设立对照组(3%质量分数淀粉溶液)和低、中、高剂量组(12.5、50.0、200.0 mg/kg)。待各组孕鼠产仔时,记录孕鼠的产仔情况,包括产仔数、活仔数、胎仔重量;母鼠哺乳结束后处死。各组子代大鼠随机抽取20只(雌雄各半)继续饲养,待6个月后处死。称取亲代母鼠主要脏器及子代生殖器湿重,计算脏器系数。利用ELISA法测定仔鼠血清中性激素水平,经过统计分析,评价生命早期西玛津染毒对仔鼠的生殖毒性。[结果]西玛津对活仔率及胎仔均重无明显的影响。孕期染毒中、高剂量组,及孕乳期染毒高剂量组仔鼠21 d存活率明显低于对照组(P<0.05)。孕期染毒和孕乳期染毒中、高剂量组F1代雄鼠睾丸系数均明显低于相应的对照组(P<0.05);低、中、高剂量的孕乳期染毒组F1代雄鼠睾丸系数均低于孕期(P<0.05)。孕乳期高剂量组F1代雌鼠的卵巢系数低于对照组(P<0.05)。两段染毒时期中,中、高剂量组F1代雄鼠睾酮均低于对照组(P<0.05);孕乳期中、高剂量组F1代雄鼠的睾酮水平均低于孕期染毒组(P<0.05)。两个染毒时期中,仅高剂量组F1代雌鼠血清17-β雌二醇水平低于各自对照组(P<0.05);仅孕乳期染毒高剂量组F1代雌鼠的17-β雌二醇水平明显低于孕期染毒的水平(P<0.05)。[结论]子代在生命早期通过母体接触西玛津对其生殖发育具有一定的毒作用。
[Objective] To investigate the reproductive toxicity of simazine in early life to offspring. [Methods] 90 adult female Sprague Dawley rats, 45 males, were caged according to 2: 1. A total of 80 pregnant rats were selected and randomly divided into 8 groups. During pregnancy and lactation, simazine was administered by gavage. Control group (3% starch solution) and low, medium and high dose groups (12.5, 50.0, 200.0 mg / kg). When the pregnant rats of each group were born, the litter size of the pregnant rats was recorded, including the number of litter size, the number of live litter size and the weight of the litter. The female rats were sacrificed after the end of lactation. Each group of offspring rats were randomly selected 20 (male and female half) to continue feeding until 6 months after sacrifice. Weigh the parent and the offspring of the main organs of the genitals wet weight, calculate the organ coefficient. Serum levels of sex hormones were measured by ELISA. After statistical analysis, the reproductive toxicity of simazine in early life to offspring rats was evaluated. [Results] Simazine had no obvious effect on the live-birth rate and the average weight of the fetus. The 21-day survival rate of pregnant and middle-aged, high-dose and high-dose pregnant pregnant rats was significantly lower than that of the control group (P <0.05). The testicular coefficient of F1 generation male rats during pregnancy and pregnant period was significantly lower than that of corresponding control group (P <0.05). The low, medium and high doses of F1 generation male Rats testis coefficient were lower than during pregnancy (P <0.05). The ovarian coefficient of the F1 generation female rats in the high-dose lactation group was lower than that in the control group (P <0.05). The levels of testosterone in F1 generation male rats in middle and high dosage groups were lower than those in control group (P <0.05) P <0.05). In the two exposure periods, the serum 17-β estradiol level of F1 generation female rats in the high-dose group was lower than that of the control group (P <0.05). Only the 17- β estradiol levels were significantly lower than during pregnancy (P <0.05). [Conclusion] Offspring have some toxic effects on their reproductive development through maternal exposure to simazine in early life.