论文部分内容阅读
目的探讨腹腔注射硫化氢(H_2S)对新生SD大鼠坏死性小肠结肠炎(NEC)的作用及可能机制。方法出生12 h内的新生SD大鼠75只,随机分为5组,正常对照组不予以干预,4个NEC组(Na Cl对照组、小剂量H_2S组、中剂量H_2S组、大剂量H_2S组)通过人工喂养-缺氧-冷刺激-脂多糖(LPS)方法制备新生鼠NEC模型,并分别予以0.1 ml Na Cl、0.1 ml含2.5 mg/kg、5.0 mg/kg、10.0 mg/kg的硫氢化钠(Na HS)溶液腹腔注射,采用酶联免疫吸附法(ELISA)检测各组新生鼠小肠组织肿瘤坏死因子α(TNF-α)水平,比较各组新生鼠生存时间、非NEC相关性死亡率、病理评分,分析H_2S对新生鼠NEC的作用及可能机制。结果正常对照组、Na Cl对照组、小剂量H_2S组、中剂量H_2S组、大剂量H_2S组小肠组织TNF-α水平分别为(11.2±2.5)、(133.9±5.5)、(93.9±4.6)、(51.5±6.7)、(60.9±8.0)pg/ml,病理学评分分别为(0.0±0.2)、(2.9±1.1)、(1.9±1.0)、(1.1±0.9)、(1.1±1.1)分。小剂量H_2S组及中剂量H_2S组小肠组织TNF-α水平显著降低(P<0.05),小肠病理学损伤程度降低(P<0.05),中剂量H_2S组NEC新生鼠生存率提高(P<0.05);但大剂量H_2S组炎症反应及病理损伤程度未减轻,生存时间反而缩短(P<0.05)。结论 H_2S在一定剂量范围内可通过抑制炎症反应减轻NEC损伤,提高NEC新生鼠生存率。
Objective To investigate the effect and possible mechanism of intraperitoneal injection of hydrogen sulfide (H 2 S) on necrotizing enterocolitis (NEC) in neonatal SD rats. Methods Thirty-five newborn Sprague-Dawley rats were randomly divided into five groups at 12 h after birth. The rats in the normal control group were not intervened. Four NEC groups (Na Cl control group, low dose H 2 S group, middle dose H 2 S group, high dose H 2 S group, ) NEC models of neonatal rats were prepared by artificial feeding-hypoxia-cold stimulation-lipopolysaccharide (LPS) method and were respectively treated with 0.1 ml NaCl and 0.1 ml sulfur containing 2.5 mg / kg, 5.0 mg / kg and 10.0 mg / Sodium hydride (NaHS) solution was intraperitoneally injected. The levels of tumor necrosis factor-α (TNF-α) in intestinal tissue of neonatal rats in each group were detected by enzyme-linked immunosorbent assay (ELISA) Rate, pathological score, analysis of H_2S on neonatal rat NEC and its possible mechanism. Results The levels of TNF-α in small intestine tissue were (11.2 ± 2.5), (133.9 ± 5.5) and (93.9 ± 4.6), respectively, in small amount of H 2 S group, middle dose H 2 S group and high dose H 2 S group. (51.5 ± 6.7) and (60.9 ± 8.0) pg / ml respectively, the pathological scores were (0.0 ± 0.2), (2.9 ± 1.1), (1.9 ± 1.0), (1.1 ± 0.9) and . The small intestine H_2S group and medium dose H_2S group had a significant decrease in TNF-α level (P <0.05), small intestine pathological lesion (P <0.05), and middle dose H_2S group (P <0.05) However, the inflammatory response and pathological damage in high-dose H 2 S group were not alleviated and the survival time was shortened (P <0.05). Conclusions H 2 S can reduce the damage of NEC and inhibit the survival rate of neonatal Neonatal rats by inhibiting inflammatory reaction in a certain dose range.