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目的 :研究 p2 7KIP1基因对肝癌细胞的细胞周期和细胞凋亡的调节作用 .方法 :采用一种可诱导性真核表达载体 pMD neo ,通过外加Zn2 + 诱导目的基因的表达 .脂质体转染法将 p2 7KIP1全长cDNA转入肝癌细胞系HCC 92 0 4中 ,检测p2 7KIP1基因的表达 ,对细胞增殖的作用及目的基因对
Objective: To study the role of p2 7KIP1 in the regulation of cell cycle and apoptosis in hepatocellular carcinoma cells.Methods: The inducible eukaryotic expression vector pMD neo was used to induce the expression of the target gene by addition of Zn2 + The p2 7KIP1 full-length cDNA was transfected into hepatocellular carcinoma cell line HCC 92 0 4 to detect the expression of p2 7KIP1 gene and its effect on cell proliferation and the target gene pair