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目的了解湖州市HIV-1型流行毒株的基因型分布情况,为艾滋病防治工作提供科学依据。方法采集204份HIV-1型抗体阳性的全血标本,从样本中提取病毒RNA,采用巢式PCR方法扩增病毒gag基因,对PCR产物进行核苷酸序列测定。应用Clustal W软件对测序结果和HIV分型参考株序列进行多序列比对和分析;使用MEGA 4.0软件进行系统进化分析。结果 204份标本有138份样品扩增出HIV-1的gag基因片段,共存在4种HIV-1亚型和重组型。其中CRF07_BC重组亚型74株(53.62%)、CRF01_AE重组亚型40株(28.99%)、B亚型12株(8.69%)、CRF08_BC重组亚型12株(8.69%)。异性、同性性传播以CRF07_BC和CRF01_AE为主;注射吸毒传播以CRF07_BC为主。结论湖州市不同感染人群中主要存在4种基因亚型,CRF07_BC已成为湖州市不同感染人群中HIV主要流行亚型,重组型毒株占优势,应加强对HIV-1流行毒株亚型变异的监测,及时调整防控策略。
Objective To understand the distribution of genotypes of HIV-1 strains in Huzhou and provide a scientific basis for AIDS prevention and treatment. Methods Totally 204 HIV-1 positive whole blood samples were collected. The viral RNA was extracted from the samples. The viral gag gene was amplified by nested PCR and the PCR products were sequenced. Multiple sequence alignments and analyzes of sequencing results and reference strains of HIV typing were performed using Clustal W software. Phylogenetic analysis was performed using MEGA 4.0 software. Results There were 138 samples of 204 samples amplified gag gene fragment of HIV-1, a total of four HIV-1 subtypes and recombinant. Among them, 74 (53.62%) were CRF07_BC, 40 (28.99%) were CRF01_AE, 12 (8.69%) were B subtype and 12 (8.69%) were CRF08_BC subtype. Heterosexual, homosexual transmission to CRF07_BC and CRF01_AE based; injection drug abuse to CRF07_BC-based. CONCLUSIONS: There are 4 subtypes of genotypes in different infected populations in Huzhou City. CRF07_BC has become the major epidemic subtype of HIV among different infected populations in Huzhou City. The recombinant strains predominate, and the subtypes of HIV-1 strains should be strengthened Monitoring, timely adjustment of prevention and control strategies.