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目的 :探讨肝脏及肝脏内Kupffer细胞在同种异系抗原门静脉接种诱导的免疫耐受机制中的作用。方法 :以NIH/q小鼠淋巴细胞对BALB/C小鼠门静脉或腔静脉接种 ,一周后再行皮下接种二次 ,间隔时间为一周 ,第二次皮下接种后一周 ,测定供受体混合淋巴细胞增生反应 (MLR)和受体对供体抗原的迟发性超敏反应 (DTH)。经神经氨酸酶 (neuraminidase)处理的供体淋巴细胞经受体小鼠下腔静脉接种 ,以及在门静脉接种前一天 ,预先用Kupffer细胞抑制剂三氯化钆 (GdCl3)溶液 ,经受体小鼠的尾静脉注射以阻断Kupffer细胞的功能 ,比较各组结果。 结果 :经神经氨酸酶处理的供体淋巴细胞经受体小鼠下腔静脉接种组 ,供受体MLR其刺激指数 (SI)为 3 6 3± 0 31,DTH脚掌厚度增值为 (7 5 0± 0 80 )× 10 -2 mm(n =7) ,与供体淋巴细胞门静脉接种组 (n =8)比较 ,结果差异不显著(P >0 0 5 )。预先GdCl3经受体小鼠尾静脉注射 ,可阻碍门静脉接种引起的免疫抑制 ,其MLR(SI)为 34 4± 1 12 ,DTH脚掌厚度增值为 (2 3 7± 1 30 )× 10 -2 mm(n =8) ,与对照组 (n =8)及下腔静脉接种组 (n =8)比较 ,结果差异不显著 (P >0 0 5 )。 结论 :受体鼠肝脏及肝脏内Kupffer细胞对门静脉注射的异基因供体淋巴细胞的选择性捕捞 ,是门静脉免疫耐受诱导?
Objective: To investigate the role of Kupffer cells in the liver and liver in the mechanism of immune tolerance induced by allogenic antigen inoculation of portal vein. Methods: NIH / q mouse lymphocytes were inoculated into the portal vein or vena cava of BALB / C mice. One week later, the mice were inoculated subcutaneously twice a week for one week. One week after the second subcutaneous inoculation, Cell Proliferation (MLR) and Delayed Hypersensitivity (DTH) of Receptor to Donor Antigen. Donor lymphocytes treated with neuraminidase were inoculated into the inferior vena cava of mice and one day prior to portal vein inoculation with a small amount of gadolinium trichloride (GdCl3) solution, a Kupffer cell inhibitor, Rat tail vein injection to block Kupffer cell function, comparing the results of each group. Results: The neuraminidase - treated donor lymphocytes were transplanted into the inferior vena cava of recipient mice. The sensitivities index (ML) of donor lymphocytes was 363 ± 0 31 and the increase of thickness of the sole of DTH was (75 0 ± 0 80) × 10 -2 mm (n = 7). There was no significant difference between the two groups (P> 0.05) in comparison with donor lymphocytic portal vein inoculation group (n = 8). Preincubation of mice with GdCl3 by tail vein injection blocked the immunosuppression induced by portal vein injections, with an MLR (SI) of 34 4 ± 1 12 and an increase in the thickness of the somatomedullary (23 7 ± 1 30) × 10 -2 mm (n = 8). There was no significant difference between the control group (n = 8) and IVC inoculation group (n = 8) (P> 0.05). Conclusion: The selective fishing of allogeneic donor lymphocytes injected by Kupffer cells in the liver and liver of recipient mice is a portal vein immune tolerance induction.