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作为第二届世界神经科学会议(布达佩斯,1987年8月16～21日)的卫星会议之一,“阿片肽的调节作用”专题讨论会于8月14～15日在布达佩斯举行。参加者100余人,论文摘要76篇,其中口头报告27篇,墙报展出49篇。会议重点讨论了μ,δ和κ等不同类型阿片受体的结构与它们的配基、各类阿片肽的生理和药理作用,以及阿片肽和其它神经递质的关系。关于阿片受体的结构,国际上有两种做法。一是以蛋白质化学的方法提纯受体蛋白,分析氨基酸组成;二是以分子生物学方法克隆受体,从基因水平上搞清受体的结构。用生化方法提纯μ和δ受体的工作进展较快,κ受体的提纯进展较慢;但目前提纯的蛋白质还不完全符合膜蛋白的要求。作为受体蛋白,穿过细胞膜的部分应该主要由疏水氨基酸组成。如果纯化的蛋白质结构上没有这样的段落,就要怀疑其是否为膜蛋白。关于阿片受体的克隆工作,英、美两国各有一些实验室在全力进行,包括英国剑桥大学的 E.Barnard,
As one of the satellite conferences for the Second World Conference of Neurosciences (Budapest, August 16-21, 1987), the Symposium on “Modulation of Opioid” was held in Budapest from August 14 to 15. More than 100 participants, 76 abstracts, including 27 oral reports and 49 poster posters. The meeting focused on the different types of μ, δ and κ opioid receptor structure and their ligand, various types of opioid physiology and pharmacological effects, as well as opioid peptides and other neurotransmitter relationship. There are two international approaches to the structure of opioid receptors. One is to purify the receptor protein by protein chemistry and analyze the amino acid composition; the other is to clone the receptor by molecular biology and get the structure of the receptor from the gene level. Purification of μ and δ receptors by biochemical methods progressed rapidly, and the progress of purification of κ receptor was slow. However, the purified proteins did not fully meet the requirements of membrane proteins. As a receptor protein, the portion that crosses the cell membrane should consist primarily of hydrophobic amino acids. If there is no such passage in the structure of the purified protein, it is doubtful if it is a membrane protein. On the opioid receptor cloning work, Britain, the United States each have some laboratories in full swing, including E. Barnard, University of Cambridge,