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目的通过对不同产地荆芥Schizonepeta tenuifolia中6种黄酮类成分的检测,为其质量控制提供依据。方法采用高效液相色谱(HPLC)法建立荆芥药材中黄酮类成分的检测方法,并以6种黄酮类成分的量为数据来源,对其进行聚类分析。结果不同产地荆芥中6种黄酮类成分的量差异较大,木犀草苷、槲皮苷、橙皮苷、木犀草素、芹菜素、香叶木素的线性范围分别是0.030~0.148μg、0.222~1.108μg、0.357~1.784μg、0.058~0.292μg、0.054~0.269μg、0.050~0.247μg,加样回收率为96.32%~99.97%,RSD<2.20%;其中,安徽产地聚为一类,河南1与广东产地为聚一类,河南2、河北、云南、四川、湖北、甘肃产地聚为一类。结论建立的方法简单、灵敏,可用于荆芥的多指标成分的质量控制;其次,河南1、河南2、河北、云南、四川、湖北、广东、甘肃的亲缘关系较近,可以作为同一药用来源。
Aim To test the quality control of six flavonoids in Schizonepeta tenuifolia from different areas. Methods The HPLC method was used to establish the method for the determination of flavonoids in Nepeta medicinal herbs. The data of six flavonoids were used as data source and clustered. Results There were significant differences in the contents of six flavonoids in different regions of Schizonepeta tenuifolia. The linear ranges of luteolin, quercitrin, hesperidin, luteolin, apigenin, and safranin were 0.030 ~ 0.148μg, 0.222 ~ 1.108μg, 0.357 ~ 1.784μg, 0.058 ~ 0.292μg, 0.054 ~ 0.269μg, 0.050 ~ 0.247μg, the recoveries were 96.32% ~ 99.97%, RSD <2.20% 1 and polygamy originated in Guangdong, Henan 2, Hebei, Yunnan, Sichuan, Hubei and Gansu. Conclusion The established method is simple and sensitive and can be used for the quality control of multi-index components of Nepeta. Secondly, the genetic relationship between Henan 1, Henan 2, Hebei, Yunnan, Sichuan, Hubei, Guangdong and Gansu is relatively close and can be used as the same medicine source.