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目的 :研究吲哚青绿 (indocyaninegreen ,ICG)溶液对体外培养的人视网膜色素上皮细胞 (retinalpigmentepitheli um ,RPE)和视网膜神经胶质细胞 (retinalglialcells ,RGC)的毒性。方法 :将ICG分别溶解于注射用水、乳酸林格氏缓冲液和无血清培养基 ,配制成 0 2 5、0 0 5、0 0 2 5mg/ml浓度的ICG溶液。测定各种溶液的渗透压与pH值。采用以上三种溶液作用于第 3代人RGC和RPE细胞 ,1、5、10分钟后更换为含 10 %小牛血清的DMEM培养基 ,37℃继续孵育 2 4小时。检测细胞形态和细胞生存率。结果 :本实验浓度的乳酸林格氏液组与无血清培养基组的ICG未引起RPE与RGC细胞形态改变 ,但随浓度和时间增加 ,两种细胞吸光度值降低 (P <0 0 5 )。低浓度注射用水组ICG溶液明显抑制细胞生存 (P <0 0 1)。结论 :ICG对人RPE和RGC细胞有一定毒性。ICG溶液对RPE和RGC细胞生长的抑制具有浓度和时间依赖性。对两种细胞活性的抑制无选择性。使用低浓度ICG ,溶解于等渗溶剂 ,缩短ICG接触时间 ,可减轻其对视网膜细胞的毒性作用。
Objective: To study the toxicity of indocyanine green (ICG) solution on human retinal pigment epithelial cells (RPE) and retinal glial cells (RGCs) cultured in vitro. Methods: ICG were dissolved in water for injection, Ringer ’s lactate buffer and serum - free medium respectively to prepare ICG solution with concentration of 0 2 5, 0 0 5, 0 0 2 5mg / ml. Determination of various solutions of osmotic pressure and pH. The above three solutions were used to act on the third generation of human RGC and RPE cells. After 1, 5 and 10 minutes, the medium was changed to DMEM medium containing 10% fetal bovine serum and incubated for 24 hours at 37 ° C. Detection of cell morphology and cell survival rate. Results: The ICG of lactated Ringer’s solution group and serum-free medium group did not cause morphological changes of RPE and RGC cells. However, the absorbance of both cells decreased with the increase of concentration and time (P <0.05). Low concentrations of water for injection ICG solution significantly inhibited cell survival (P lt; 0 01). Conclusion: ICG has some toxicity on human RPE and RGC cells. The ICG solution inhibited the growth of RPE and RGC cells in a concentration and time-dependent manner. No selectivity for inhibition of both cell activities. The use of low concentrations of ICG, dissolved in isotonic solvent, shorten the ICG contact time, can reduce its toxic effects on retinal cells.