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目的 :用高效液相色谱梯度洗脱法同时检测血塞通滴丸中三七皂苷R1、人参皂苷Rb1、人参皂苷Rg13种皂苷含量的方法 ,为制定质量标准中含量测定方法及含量限度提供了依据。方法 :用大连SpherisorbC18分析柱 ,乙腈 水线性梯度洗脱 ,0～ 15min(2 0∶80 - 4 0∶6 0 ) ,流速 1.0mL·min-1,检测波长 2 0 3nm。结果 :R1,Rf1和Rb1线性范围分别为 1.0 2～ 9.18μg ,4 .8～ 4 3.4 μg和 4 .6～ 4 1.6 μg。该方法回收率R1为 10 1.0 % (RSD =3.18% ) ,Rb1为 10 0 .0 % (RSD =1.19% ) ,Rg1为 99.5 % (RSD =3.0 2 % )。结论 :HPLC梯度洗脱法能将多种皂苷很好地分离检测 ,提高了时效 ,减少了误差 ,结果表明该方法准确可靠 ,重现性好 ,结果稳定
Objective: To detect the contents of notoginsenoside R1, ginsenoside Rb1, and ginsenoside Rg13 saponin in Xuesaitong Dripping Pill by high performance liquid chromatography gradient elution method, and to provide a method for determination of content and limit of content in quality standard. in accordance with. METHODS: A Dalian Spherisorb C18 analytical column with linear gradient elution of acetonitrile and water was used. The linear gradient elution was 0～15min (2:0:80 - 4:0:60), the flow rate was 1.0mL·min-1, and the detection wavelength was 203 nm. Results: The linear ranges of R1, Rf1 and Rb1 were 1.0 2 to 9.18 μg, 4.8 to 4 3.4 μg and 4.6 to 4 1.6 μg, respectively. The recoveries of this method were R1 of 10 1.0 % (RSD = 3.18%), Rb1 of 10 0.0% (RSD = 1.19%) and Rg1 of 99.5 % (RSD = 3.0 2 %). Conclusion : The HPLC gradient elution method can separate and detect various saponins, improve the time efficiency and reduce the error. The results show that the method is accurate and reliable, and the reproducibility is good. The results are stable.