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过去曾报导,癌症患者血清总半乳精转移酶(GT)活性升高,但这种升高既不一致又无特异性。有人报导血清GT的一种同工酶与肿瘤细胞有特定的联系,但这一发现由于电泳技术上的困难未得到进一步证实。本文作者取新鲜血清在含有两性电解质载体(pH4～6)的等电聚焦柱中进行分析,研究了正常人、非癌肝病患者和肝癌患者的血清GT活性的等电聚焦分型。实验发现,四例正常人的血清GT活性在等电聚焦中产生三个峰,其等电点大约在pH4.8、4.95
It has been reported in the past that serum total galactotransferase (GT) activity is elevated in cancer patients, but this increase is both inconsistent and non-specific. It has been reported that an isoenzyme of serum GT has a specific association with tumor cells, but this finding has not been further confirmed due to technical difficulties in electrophoresis. The authors took fresh serum for analysis on an isoelectric focusing column containing ampholytes (pH 4-6) to study the isoelectric focusing of serum GT activity in normal people, non-cancerous liver disease patients, and liver cancer patients. It was found that the serum GT activity in four healthy individuals produced three peaks in isoelectric focusing, with isoelectric points at pH 4.8, 4.95