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现已确定除了细菌产物、吞噬刺激、免疫调节剂外,一些非免疫因素如神经肽类也能调节单核吞噬细胞的功能,例如神经降压素(Neurotensin,NT)能提高腹腔巨噬细胞的吞噬功能及细胞毒作用。NT 广泛存在于肺尤其是与下呼吸道血管密切相关的神经纤维内,为了更好地了解肺微环境中免疫功能的调节,本文作者研究了NT 对肺泡巨噬细胞(AM)产生IL-1活性的影响。取250—300gWistar 大鼠,通过支气管肺泡灌洗制得AM,将AM(0.5×10~6/ml)与不同的诱导剂(如LPS、MDP,酵母多糖、NT、LTB_4、IFN-γ)孵育24hr 后,经离心
It has been determined that in addition to bacterial products, phagocytic stimuli, and immunomodulators, some non-immune factors such as neuropeptides can modulate the function of mononuclear phagocytes, such as neurotensin (NT), which increases peritoneal macrophages Phagocytosis and cytotoxicity. NT is widely found in the lungs, especially in the nerve fibers that are closely related to the lower respiratory tract. To better understand the regulation of immune function in the lung microenvironment, the authors studied the effect of NT on IL-1 production by alveolar macrophages (AM) Impact. 250-300 g Wistar rats were taken and AM was made by bronchoalveolar lavage. AM (0.5 × 10 -6 / ml) was incubated with different inducers (such as LPS, MDP, zymosan, NT, LTB_4, IFN-γ) After 24 hr, centrifuge