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目的 检测了 65例NIDDM患者中性粒细胞 (PMN)膜流动性 (MF)的改变 ,探讨这种改变与年龄、病程、BMI、FBG、PBG、TG、TC、WBC、RBC、PLT、GPI的相关关系。方法 65例无酮症及感染的NIDDM患者 ,3 0例性别、年龄、BMI相匹配的正常人为对照 ,以DPH为探针测定其PMN的荧光偏振值 ( ρ) ,并计算相应的细胞膜流动性 ,膜脂各向异性及微粘度。结果 NIDDM患者的PMNρ值、膜脂各向异性及微粘度均显著高于对照组 (P <0 .0 1) ,PMNMF显著低于对照组 (P <0 .0 5 )。PMNMF值与FBS、PBS、GPI水平呈负相关 (r = 0 .3 4 17,r = 0 .3 2 3 7,r = 0 .3 913P <0 .0 5 ) ,与年龄、病程、BMI、WBC、RBC、PLT、TG、TC无相关性。结论 NIDDM患者PMN的MF下降 ,提示白细胞膜的物理性质改变 ,PMNMF下降与血糖升高、糖化血清蛋白增加有关。
Objective To investigate the changes of membrane fluidity (MF) of neutrophil (PMN) in 65 patients with NIDDM and to explore the relationship between this change and age, course of disease, BMI, FBG, PBG, TG, TC, WBC, RBC, PLT, GPI relationship. Methods Thirty 65 NIDDM patients without ketosis and infection were enrolled in this study. Twenty-three normal controls of gender, age and BMI were used as control. The fluorescence polarization (ρ) of PMN was measured by DPH probe and the corresponding membrane fluidity , Lipid anisotropy and micro-viscosity. Results The values of PMNp, membrane lipid anisotropy and microviscosity in patients with NIDDM were significantly higher than those in the control group (P <0.01). PMNMF was significantly lower than that in the control group (P <0.05). The values of PMNMF were negatively correlated with the levels of FBS, PBS and GPI (r = 0.3417, r = 0.3237, r = 0.3913P <0.05) WBC, RBC, PLT, TG, TC no correlation. Conclusion The decrease of MF of PMN in patients with NIDDM suggests that the physical properties of leukocyte membrane are changed. The decrease of PMNMF is associated with the increase of blood glucose and the increase of glycated serum protein.