论文部分内容阅读
为了探讨小菜蛾Plutella xylostella血淋巴对玫烟色棒束孢Isaria fumosorosea的防御机制,利用吉姆萨染色法在光镜下观察了小菜蛾4龄幼虫血细胞感染不同致病力玫烟色棒束孢后的免疫反应。结果表明:玫烟色棒束孢的入侵可导致小菜蛾血细胞数量发生改变,表现为入侵初期血细胞总数增加,不同类型血细胞比例改变等。体表接种后8-45h,高致病力菌株PFCF-001处理的幼虫血细胞总数在24h出现最高值6250个/mm3,而低致病力菌株PFCF-D58处理在36h达到最高值3000个/mm3,比高致病力菌株处理滞后12h。不同菌株处理下虫体参与防御反应的主要血细胞类型为浆血细胞和粒血细胞。小菜蛾幼虫血细胞在感菌初期能够产生粘附、吞噬、包被及形成结节等一系列防卫反应,但最终无法抵挡高致病力菌株PFCF-001的侵染。结果说明小菜蛾幼虫血淋巴对玫烟色棒束孢的防御反应只有短暂的抑制作用,不能从根本上清除、消灭玫烟色棒束孢。
In order to investigate the defense mechanism of the Plutella xylostella hemolymph to Isaria fumosorosea, the 4th instar larvae of Plutella xylostella hemolymph were infected with different virulence factors Immune response. The results showed that the invasion of C. obliquus could lead to the change of blood cell numbers in Plutella xylostella, showing the increase of the total number of blood cells and the change of the proportion of different types of blood cells in the early stage of invasion. The total number of larval blood cells in the PFCF-001-treated larvae reached a maximum of 6250 cells / mm3 at 8-45 h after inoculation, while the highest pathogenicity of PFCF-D58 at 3000 hPa / mm3 , Than the high virulence strains lag 12h. The main types of blood cells involved in the defense response of the parasites under different bacterial strains were plasma cells and granulocytes. Plutella xylostella larval blood cells in the initial susceptible to adhesion, phagocytosis, coating and the formation of nodules and a series of defense response, but ultimately can not resist the infection of the highly pathogenic strain PFCF-001. The results showed that the larvae of Plutella xylostella larvae had a short-lasting inhibitory effect on the defense response of C. obliquus, and could not be completely eliminated and eliminated.