目的:探讨胃癌组织果糖-1,6-二磷酸酶(FBP1)表达及其对生存预后的预测价值。方法:收集81例行手术治疗的胃癌患者胃癌组织和癌旁组织,另收集65例胃良性病变患者正常胃黏膜组织(正常组织),采用免疫组织化学方法检测各组织FBP1蛋白表达,实时定量聚合酶链反应(RT-PCR)检测组织FBP1 mRNA表达;收集胃癌患者临床病理资料,分析FBP1表达水平与胃癌病理资料的关系;对胃癌患者进行随访,分析FBP1表达与患者生存率的关系。结果:胃癌组织FBP1蛋白高表达率为27.2%(22/81),癌旁组织为56.8%(46/81),正常组织为83.1%(54/65),胃癌组织FBP1蛋白高表达率显著低于癌旁组织和正常组织(P<0.05);胃癌组织FBP1蛋白染色评分亦显著低于癌旁组织和正常组织(P<0.05)。FBP1 mRNA相对表达量胃癌组织为(0.294±0.048),癌旁组织为(0.531±0.067),正常组织为(0.935±0.098),胃癌组织显著低于癌旁组织和正常组织(P<0.05)。胃癌组织FBP1表达水平与肿瘤直径、TNM分期、淋巴结转移有关,肿瘤直径越大、TNM分期越高、合并淋巴结转移的胃癌患者FBP1低表达率越高(P<0.05)。胃癌患者FBP1高表达组3年累积生存率为77.4%,FBP1低表达组3年累积生存率为43.8%,FBP1高表达组显著高于低表达组(P<0.05)。结论:胃癌组织FBP1表达显著降低,其表达水平与肿瘤大小、临床分期、淋巴结转移有关;FBP1表达越高,胃癌患者生存率越高,FBP1可以作为胃癌病情和预后的预测指标之一。 Objective: To investigate the expression of fructose-1,6-bisphosphatase (FBP1) and its prognostic value in gastric cancer. Methods: Totally 81 gastric cancer tissues and adjacent non-cancerous tissues were collected from gastric cancer patients. Normal gastric mucosa tissues (normal tissues) were collected from 65 patients with benign gastric lesions. The expression of FBP1 protein was detected by immunohistochemical method. The clinicopathological data of gastric cancer patients were collected to analyze the relationship between FBP1 expression and pathological data of gastric cancer. The patients with gastric cancer were followed up to analyze the relationship between FBP1 expression and survival rate. Results: The high expression rate of FBP1 protein was 27.2% (22/81) in gastric cancer tissues, 56.8% (46/81) in paracancer tissues and 83.1% (54/65) in normal tissues. The high expression rate of FBP1 protein in gastric cancer tissues was significantly lower (P <0.05). The staining score of FBP1 protein in gastric cancer tissues was significantly lower than that in paracancerous tissues and normal tissues (P <0.05). The relative expression of FBP1 mRNA in gastric cancer tissue was (0.294 ± 0.048), adjacent tissue (0.531 ± 0.067), normal tissue (0.935 ± 0.098), gastric cancer tissue was significantly lower than that in paracancerous tissues and normal tissues (P <0.05). The expression of FBP1 in gastric cancer tissue was correlated with tumor diameter, TNM stage and lymph node metastasis. The larger the tumor diameter, the higher the TNM stage, the higher the FBP1 expression in gastric cancer with lymph node metastasis (P <0.05). The 3-year cumulative survival rate was 77.4% in FBP1-overexpressing group, 3-year cumulative survival rate was 43.8% in FBP1-overexpressing group, and significantly higher in FBP1-overexpressing group than in low expression group (P <0.05). Conclusions: The expression of FBP1 in gastric cancer is significantly decreased. The expression level of FBP1 is correlated with tumor size, clinical stage and lymph node metastasis. The higher the FBP1 expression is, the higher the survival rate of gastric cancer patients is. FBP1 may be used as one of the prognostic indicators of gastric cancer.