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用 HPLC法测定三磷酸腺苷二钠滴眼液中三磷酸腺苷二钠的含量,以 Hypersil ODS2为分析柱,磷酸盐缓冲液(PH 7. 0)为流动相,检测被长为 259 nm,三磷酸腺苷二钠在 p=1.5-96μg/mL范围内与峰面积呈良好的线性关系(r=0.9998),三磷酸腺苷二钠平均回收率为99.91%,RSD为0.25%。本法适用于该制剂的质量控制。
The content of adenosine triphosphate (ATP) in sodium adenosine triphosphate (DPA) eye drops was determined by HPLC. Hypersil ODS2 was used as the analytical column and phosphate buffer (pH 7.0) was used as the mobile phase. = 1.5-96μg / mL range and the peak area showed a good linear relationship (r = 0.9998), the average recovery of adenosine triphosphate was 99.91%, RSD 0.25%. This Law applies to the quality control of the preparation.