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目的:为给杂交育种和人工辅助授粉提供参考。方法:用花粉离体培养法,研究了不同植物生长调节剂对泰国白豆蔻花粉萌发和花粉管生长的影响。结果:不同植物生长调节物质对花粉萌发和花粉管生长的作用不同。6-苄氨基嘌呤(6-BA)、赤霉素对白豆蔻花粉萌发及花粉管生长均表现较强的抑制作用;萘乙酸在0.5 mg·L-1时不仅有利于花粉萌发而且有利于花粉管生长;硼酸在100～200 mg·L-1质量浓度范围内促进白豆蔻花粉的萌发和花粉管的生长,最适质量浓度为100 mg·L-1;多效唑对白豆蔻花粉萌发起抑制作用,抑制程度随质量浓度的提高而减弱,促进白豆蔻花粉萌发时,可选择800 mg·L-1的多效唑质量浓度。结论:生产中在花期喷施一定浓度萘乙酸、硼酸或多效唑,可促进花粉萌发和花粉管生长,提高泰国白豆蔻的坐果率。
Objective: To provide reference for hybrid breeding and artificial pollination. Methods: The effects of different plant growth regulators on the pollen germination and pollen tube growth of Thai nutmeg were studied by in vitro culture method. Results: The effects of different plant growth regulators on pollen germination and pollen tube growth were different. 6-benzylaminopurine (6-BA) and gibberellin showed a strong inhibitory effect on pollen germination and pollen tube growth. NAA at 0.5 mg · L-1 was not only beneficial to pollen germination but also to pollen tube The results showed that boric acid could promote the germination of pollen and the growth of pollen tube in the concentration range of 100-200 mg · L-1 with the optimal concentration of 100 mg · L-1. PP333 could inhibit the germination of S. cardamom pollen, The degree of concentration was weakened with the increase of the mass concentration, and the concentration of paclobutrazol at the concentration of 800 mg · L-1 was selected to promote the germination of the white cardamom pollen. Conclusion: The application of naphthalene acetic acid, boric acid or paclobutrazol during the flowering stage can promote pollen germination and pollen tube growth, and improve the fruit setting rate of Thailand nutmeg.