论文部分内容阅读
目的:探讨STAT3、p-STAT3蛋白在甲状腺乳头状癌(PTC)中的表达及其与上皮间质转化的关系及意义。方法:采用免疫组织化学方法检测56例PTC组织中STAT3、p-STAT3蛋白及上皮标志物E-cadherin和间质标志物Vimentin蛋白的表达,分析其与PTC临床病理特征间的关系及相互之间的相关性。结果:PTC组织中STAT3、p-STAT3蛋白的阳性表达率为78.6%和83.9%,明显高于癌旁甲状腺组织中的阳性表达率33.3%和20.8%(P<0.01)。PTC组织中E-cadherin的阳性表达率为37.5%,明显低于癌旁甲状腺组织中的阳性表达率91.7%(P<0.01),PTC组织中Vimentin蛋白的阳性表达率为85.7%,明显高于癌旁甲状腺组织中的阳性表达率8.3%(P<0.01)。STAT3、p-STAT3、E-cadherin、Vimentin蛋白的表达与性别、年龄无明显相关性(P>0.05),而均与PTC淋巴结转移、临床分期明显相关(P<0.05)。STAT3、p-STAT3蛋白的表达和E-cadherin蛋白的表达呈负相关(r=-0.494,r=-0.364,均P<0.01),STAT3、p-STAT3蛋白的表达与Vimenin蛋白的表达呈正相关(r=0.533,P=0.000;r=0.377,均P<0.01)。结论:PTC组织中存在STAT3蛋白活化及EMT,且与PTC淋巴结转移密切相关;STAT3通路激活可能通过介导PTC细胞EMT促进PTC侵袭转移。
Objective: To investigate the expression of STAT3 and p-STAT3 protein in papillary thyroid carcinoma (PTC) and their relationship with epithelial-mesenchymal transition. Methods: Immunohistochemistry was used to detect the expression of STAT3, p-STAT3 protein and epithelial marker E-cadherin and interstitial markers Vimentin protein in 56 cases of PTC. The relationship between them and clinicopathological features of PTC was analyzed Relevance. Results: The positive rates of STAT3 and p-STAT3 in PTC tissues were 78.6% and 83.9%, respectively, which were significantly higher than those in parathyroid tissue (33.3% and 20.8%, P <0.01). The positive expression rate of E-cadherin in PTC tissues was 37.5%, which was significantly lower than that in adjacent thyroid tissues (91.7%, P <0.01). The positive expression rate of Vimentin protein in PTC tissues was 85.7% The positive expression rate in parathyroid tissue was 8.3% (P <0.01). The expression of STAT3, p-STAT3, E-cadherin and Vimentin had no significant correlation with gender and age (P> 0.05), but all had significant correlation with PTC lymph node metastasis and clinical stage (P <0.05). There was a negative correlation between the expression of STAT3 and p-STAT3 protein and the expression of E-cadherin protein (r = -0.494, r = -0.364, all P <0.01) (r = 0.533, P = 0.000; r = 0.377, all P <0.01). Conclusion: STAT3 protein activation and EMT exist in PTC tissues, which are closely related to PTC lymph node metastasis. Activation of STAT3 pathway may promote the invasion and metastasis of PTC by mediating the EMT of PTC cells.