论文部分内容阅读
建立啤酒大麦品种甘啤4号成熟胚再生体系可为其遗传转化奠定基础。本研究以甘啤4号成熟胚为材料,研究不同诱导培养基对其愈伤组织诱导及分化的影响,并通过添加不同浓度梯度的Ag NO3(0,2.5 mg/L,5.0 mg/L,7.5 mg/L,10.0 mg/L,15.0 mg/L)和ABA(0,0.1 mg/L,0.3 mg/L,0.5 mg/L,0.7 mg/L,1.0 mg/L),观察其愈伤组织形态变化,统计出愈率、绿点率,探索适宜甘啤4号成熟胚组织培养的条件。同时在0、7 d、14 d、21 d和二叶期分别测定成熟胚再生过程中SOD、POD、CAT活性、MAD、可溶性蛋白及内源激素(IAA,ABA,GA3)的含量,为优化成熟胚再生体系准备条件。研究结果表明:培养基NMB较适宜甘啤4号成熟胚再生培养,发现添加适量Ag NO3和ABA有助于愈伤组织的诱导及分化,但Ag NO3处理的效果较ABA明显,其诱导甘啤4号愈伤组织的最适浓度分别为7.5 mg/L和0.5 mg/L;观察到不同时期酶活性大致均呈现先上升后下降的趋势,内源激素含量也随着培养时期的不同有所差异。研究结果可为添加外源激素、进一步优化培养条件奠定了基础。
The establishment of beer barley varieties Ganpi No.4 mature embryo regeneration system laid the foundation for its genetic transformation. In this study, the mature embryos of Ganpi No.4 were used as materials to study the effects of different induction mediums on callus induction and differentiation. AgNO3 (0,2.5 mg / L, 5.0 mg / L, 7.5mg / L, 10.0mg / L, 15.0mg / L) and ABA (0,0.1 mg / L, 0.3 mg / L, 0.5 mg / L, 0.7 mg / L, 1.0 mg / L) Histological changes, the rate of statistics, the green point rate, to explore the suitable conditions for Ganpi 4 mature embryo tissue culture. At the same time, the activities of SOD, POD, CAT, MAD, soluble protein and endogenous hormones (IAA, ABA, GA3) in mature embryos were measured at 0, 7 d, 14 d, Mature embryo regeneration system preparation conditions. The results showed that medium NMB was more suitable for regeneration of mature embryos of Ganpi No.4 and found that addition of proper amount of AgNO3 and ABA could promote the induction and differentiation of callus. However, the effect of AgNO3 treatment was more obvious than that of ABA, The optimal concentration of callus of No. 4 was 7.5 mg / L and 0.5 mg / L, respectively. The enzyme activities in different stages showed the trend of increasing firstly and then decreasing, and the content of endogenous hormones also varied with different culture periods difference. The results can lay the foundation for adding exogenous hormones and further optimizing culture conditions.