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目的 探讨高氧暴露对体外培养的早产鼠肺泡Ⅱ型细胞 (AECⅡ )表面活性物质蛋白(surfactantassociatedprotein ,SP)A、B、CmRNA表达的影响及苦杏仁甙的作用。 方法 分离、培养19d早产鼠AECⅡ ,经贴壁纯化后随机分为四组 :空气组 (Ⅰ组 )、高氧组 (Ⅱ组 )、空气 +苦杏仁甙组(Ⅲ组 )、高氧 +苦杏仁甙组 (Ⅳ组 )。Ⅱ、Ⅳ组在更换培养液后按 3L/min通入 95 %氧、5 %二氧化碳高纯混合气 ,10min后密封。Ⅲ、Ⅳ组在更换培养液时加入 2 0 0 μmol/L苦杏仁甙 ,培养 2 4h后 ,收获各组细胞 ,Trizol一步法提取细胞总RNA ,RT PCR半定量分析各组培养细胞中SP A、SP B、SP CmRNA表达强度。 结果 Ⅱ组SP A、SP B、SP CmRNA表达强度均较Ⅰ组弱 (P <0 .0 1) ,Ⅲ组与Ⅰ组比较差异无显著性 (P >0 .0 5 ) ,Ⅳ组较Ⅱ组表达强度明显增加 (P <0 .0 5 )。 结论 高氧暴露导致早产鼠AECⅡSP A、SP B、SP CmRNA表达降低 ,这种改变参与了高氧肺损伤的发病过程 ,苦杏仁甙可抑制这种变化 ,提示苦杏仁甙对高氧肺损伤起一定的保护作用。
Objective To investigate the effects of hyperoxia exposure on the expression of surfactant-associated protein (SP) A, B, and C mRNA in alveolar type II cells (AECII) cultured in premature rats and the effect of amygdalin. Methods AECII isolated and cultured in premature rats for 19 days was randomly divided into four groups after adherence purification: air group (group I), hyperoxia group (group II), air + amygdalin group (group III), hyperoxia + bitterness Almond bran group (IV group). In groups II and IV, after replacing the culture solution, 95% oxygen and 5% carbon dioxide high-purity gas mixtures were introduced at 3 L/min and sealed after 10 min. In groups III and IV, 200 μmol/L amygdalin was added to the culture medium. After 24 hours of culture, the cells were harvested. Total RNA was extracted by Trizol in one step. Semi-quantitative RT-PCR analysis of SP A in cultured cells. , SP B, SP C mRNA expression intensity. Results The expression intensity of SP A, SP B, and SP C mRNA in group II was weaker than that in group I (P < 0.01). There was no significant difference between group III and group I (P > 0.05). The expression intensity of the group was significantly increased (P < 0.05). Conclusion Hyperoxia exposure leads to decreased expression of AECIISP A, SP B, and SP C mRNA in preterm rats. This change is involved in the pathogenesis of hyperoxia-induced lung injury. Amygdalin can inhibit this change, suggesting that amygdalin has effects on hyperoxia-induced lung injury. A certain degree of protection.