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为了研究HFRSV的吸入感染性和动物与动物间空气传播感染,用TK_2型气溶胶发生器发生H8205株病毒气溶胶,引入动态气溶胶暴露装置并对实验动物进行暴露.以AGI微生物气溶胶采样器进行采样,以Vero-E_6细胞空斑法分析病毒含量,对病毒耐气溶胶化和吸入病毒剂量进行了测定.对染毒动物产生气溶胶引起交叉感染能力进行了观察.结果表明:HFRS病毒雾化30min回流液病毒存活50%,AGI冲击5分钟其效价下降不明显.数种动物对该病毒气溶胶比较敏感.在本实验装备和条件下其气溶胶乳小鼠吸入0.065pfu全部感染;离乳小鼠吸入0.72pfu几乎全部感染;乳小鼠半数吸入致死量(ILD50)为0.57pfu.从乳小鼠脏器组织分离出了HFRS病毒.结果认为,气溶胶传播是HFRS流行的重要危险因素.
In order to study the inhalation infectivity of HFRSV and the airborne infection between animals and animals, the H8205 strain virus aerosol was generated by TK2 type aerosol generator and introduced into the dynamic aerosol exposure device and exposed to the experimental animals. The AGI microbial aerosol sampler The virus content was analyzed by Vero-E 6 cell plaque assay, and aerosol aerosolization and inhalation virus dose were measured.The aerosol-causing cross-infection ability of the infected animals was observed.The results showed that HFRS virus fog The 30min reflux virus virus survival 50%, AGI impact 5 minutes its titer decline was not obvious.A few animals on the virus aerosol sensitive.In the experimental equipment and conditions its aerosol milk mice inhaled 0.065pfu infection; Infected mice received almost all infections with 0.72pfu; the half-dose lethal dose (ILD50) of the mice was 0.57pfu. The HFRS virus was isolated from the tissues of the mouse mammary gland. The results showed that aerosol spread was an important risk of the epidemic of HFRS factor.