A study on knowledge transfer in a mutli-agent orga-nization is performed by applying the basic principle in physics such as the kinetic theory. Based on the theoretical analysis of the knowledge accumulation process and knowledge transfer at-tributes, a special type of knowledge field (KF) is introduced and the knowledge diffusion equation (KDE) is developed. The evolu-tion of knowledge potential is modeled by lattice kinetic equation and verified by numerical experiments. The new equation-based modeling developed in this paper is meaningful to simulate and predict the knowledge transfer process in firms. The development of the lattice kinetic model (LKM) for knowledge transfer can con-tribute to the knowledge management theory, and the managers can also simulate the knowledge accumulation process by using the LKM.