论文部分内容阅读
目的:探讨第10染色体同源丢失性磷酸酶-张力蛋白酶基因(PTEN)、Ki67在非小细胞肺癌组织中的表达及其相关性。方法:用免疫组化方法检测67例非小细胞肺癌组织以及41例癌旁正常肺组织中PTEN基因、Ki67蛋白的表达,并分析其与各临床病理指标及细胞增殖之间的相关性。结果:PTEN基因在67.16%(45/67)的非小细胞肺癌中阳性表达率为32.84%(22/67),而在正常肺组织中阳性表达率为82.97%,显著高于非小细胞癌组织。PTEN基因的表达与组织类型、细胞分化程度、淋巴结转移密切相关(分别为X2=5.44,P=0.019;X2=4.740,P=0.029;X2=4.5l,P=0.034),在鳞癌和低分化癌中PTEN基因失表达率或表达减少率较高。肺癌中Ki67的过度表达与肺癌的临床分期、淋巴结转移相关(X2=6.90,P=0.009;X2=5.68,P=0.017),PTEN蛋白阳性表达与Ki67负相关(r=-0.239,P<0.05)。细胞增殖指数越高,PTEN基因表达越少,二者呈负相关(r=-0.252,P<0.05)。结论:PTEN蛋白表达的缺失与非小细胞肺癌的恶性侵袭有关。联合检测PTEN与Ki67的表达可有助于判断非小细胞肺癌的预后。
Objective: To investigate the expression and correlation of PTEN and Ki67 in non-small cell lung cancer. Methods: The expressions of PTEN and Ki67 in 67 NSCLC tissues and 41 adjacent normal lung tissues were detected by immunohistochemistry. The correlations between the expression of PTEN and Ki67 protein and clinicopathological parameters and cell proliferation were analyzed. Results: The positive expression rate of PTEN gene in non-small cell lung cancer (67.16%) was 32.84% (22/67), while it was 82.97% in normal lung tissue, which was significantly higher than that in non-small cell carcinoma organization. The expression of PTEN gene was closely related to the type of tissue, cell differentiation and lymph node metastasis (X2 = 5.44, P = 0.019; X2 = 4.740, P = 0.029; Differentiated carcinoma PTEN gene loss of expression rate or a higher rate of reduction. The overexpression of Ki67 in lung cancer was correlated with the clinical stage and lymph node metastasis of lung cancer (χ2 = 6.90, P = 0.009; X2 = 5.68, P = 0.017) ). The higher the cell proliferation index, the less PTEN gene expression, the two was negatively correlated (r = -0.252, P <0.05). Conclusion: The loss of PTEN protein expression is associated with the malignant invasion of non-small cell lung cancer. Joint detection of PTEN and Ki67 expression can help determine the prognosis of non-small cell lung cancer.