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目的 :检测SLE患儿谷胱甘肽转移酶 μ(GSTμ)基因缺失程度及血中一氧化氮 (NO)、脂质过氧化物(LPO)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH px)和谷胱甘肽 (GSH)含量 ,分析其与SLE发病之间的关系。方法 :用PCR法检测 2 2例SLE患儿和 2 3例健康对照儿童的GSTμ基因 ,用化学分析法测上述 5项指标。结果 :SLE患儿GSTμ基因缺失率达 90 .91% ,明显高于对照组的 5 6.5 2 %。SLE活动期NO( 77.4 3± 15 .19)μmol/L、LPO( 9.95± 1.84 ) μmol/L ,明显高于稳定期和对照组的水平。SLE活动期SOD( 12 2 0 .76± 3 0 2 .81) μU/L、GSH -px( 80 .0 4± 2 4 .4 5 )U/mg、GSH( 0 .4 1± 0 .0 5 )mg/g ,明显低于稳定期及对照组水平。在SLE稳定期GSH( 0 .95± 0 .2 0 )mg/g ,仍明显低于对照组水平。血清NO水平与LPO呈直线正相关 ,与SOD、GSH Px、GSH呈直线负相关。抗ds DNA与NO、LPO呈直线正相关。结论 :GSTμ基因缺失可能是SLE发病的遗传因素之一 ,SLE活动期患者存在氧化损伤及抗氧化能力下降 ,且NO、LPO、SOD、GSH px可作为判断SLE病情变化的一组重要指标 ,提示对SLE患儿应给予抗氧化治疗。目的 :检测SLE患儿谷胱甘肽转移酶 μ(GSTμ)基因缺失程度及血中一氧化氮 (NO)、脂质过氧化物(LPO)、超氧化物歧化酶
Objective: To detect the extent of glutathione transferase μ (GSTμ) gene deletion and the levels of blood nitric oxide (NO), lipid peroxidation (LPO), superoxide dismutase (SOD), glutathione Peptides peroxidase (GSH px) and glutathione (GSH) content, analysis of its relationship with the pathogenesis of SLE. Methods: GSTμ gene was detected by PCR in 22 children with SLE and 23 healthy children. The above five indexes were measured by chemical analysis. Results: The deletion rate of GSTμ gene in children with SLE was 90.91%, which was significantly higher than that of the control group (6.562%). The activity of SLE was 77.4 3 ± 15.19 μmol / L and 9.95 ± 1.84 μmol / L, respectively, which was significantly higher than that of the control group. The activity of SLE in active phase (12 20.76 ± 3.082.81) μU / L, GSH-px (80.044 ± 2.4 4.5) U / mg and GSH (0.4 1 ± 0 .0 5) mg / g, which was significantly lower than that of the control group. GSH (0.95 ± 0.2. 0) mg / g in the stationary phase of SLE was still significantly lower than that of the control group. Serum NO level was positively correlated with LPO, negatively correlated with SOD, GSH Px and GSH. Anti-ds DNA was positively correlated with NO and LPO. CONCLUSION: Deletion of GSTμ gene may be one of the genetic factors in the pathogenesis of SLE. Oxidative damage and anti-oxidation ability of patients with active SLE may be decreased. NO, LPO, SOD and GSH-px may be used as a group of important indicators to determine the severity of SLE. SLE children should be given anti-oxidative treatment. Objective: To detect the extent of glutathione transferase μ (GSTμ) gene deletion and the levels of blood nitric oxide (NO), lipid peroxides (LPO), superoxide dismutase