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Objective:To construct a novel plasmid as Salmonella enterica serovar typhimurium(S.typhimurium)sip C gene knockouts candidate.Methods:In this research,50upstream and 30downstream regions of S.typhimurium sip C gene and kanamycin gene were PCR amplified.Each of these DNA fragment was cloned into p GEM T-easy vector.The construct was confirmed by PCR and restriction digest.Results:PCR amplified 320,206 and 835 bp DNA fragments were subcloned into p ET-32 vector resulting with a plasmid called p ET-32-sip C up-kan-sip C down.Conclusions:The new plasmid(p ET-32-sip C up-kan-sip C down)is useful for genetic engineering and for future manipulation of S.typhimurium sip C gene.
Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sip C gene knockouts candidate. Methods: In this research, 50upstream and 30downstream regions of S. typhimurium sip C gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into p GEM T-easy vector. The construct was confirmed by PCR and restriction digest. Results: PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into p ET-32 vector resulting with plasmid called p ET-32- sip C up-kan-sip C down.Conclusions: The new plasmid (p ET-32-sip C up-kan-sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sip C gene.