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目的观察表皮生长因子(EGF)及其受体(EGFR)在Nitrofen诱导的CDH模型胎肺中表达的情况,探讨CDH时肺发育不良的原因。方法实验组20只SD怀孕大鼠于孕9.5d时经胃管给予Nitrofen,正常对照组给食用油,孕21.5d时对所有孕鼠行剖宫产,取出胎鼠两侧肺组织进行EGF和EGFR免疫组化染色和图像分析。结果实验组死亡1只,致畸率46.7%;CDH肺发育不良,处于假腺体期和原始肺小管期,EGF表达上调而EGFR表达下调(P<0.05),膈疝侧与非膈疝侧肺组织EGF及EGFR表达差异没有统计学意义(P>0.05);实验组内产生CDH者胎肺内EGF和EGFR表达与无CDH者相差不大(P>0.05)。结论大鼠CDH模型中,腹腔内器官进入胸腔对肺组织的压迫可能并不是肺发育不良的主要原因,肺发育不良于膈疝形成前就已发生。CDH肺发育不良与EGF EGFR系统的变化密切相关。
Objective To observe the expression of epidermal growth factor (EGF) and its receptor (EGFR) in the fetal lung of Nitrofen induced CDH model and to explore the causes of lung dysplasia during CDH. METHODS: Twenty SD pregnant rats in experimental group were given Nitrofen by gastric tube at 9.5 d of pregnancy, and the normal control group were given oil. Cesarean section was performed on all pregnant rats at 21.5 d of pregnancy. The lung tissues on both sides of fetus were removed for EGF and EGFR immunohistochemical staining and image analysis. Results In the experimental group, 1 died and the teratogenic rate was 46.7%. CDH lung dysplasia was in the period of gigantic period and primary pulmonary tubule. The expression of EGF was up-regulated and the expression of EGFR was down-regulated (P <0.05) There was no significant difference in the expression of EGF and EGFR between the two groups (P> 0.05). The expression of EGF and EGFR in the fetal lung tissue in the experimental group was similar to those without CDH (P> 0.05). Conclusions In rat CDH model, the compression of lung tissue by the intrathoracic organ into the thorax may not be the main cause of pulmonary dysplasia. Lung dysplasia occurs before the diaphragmatic hernia is formed. CDH lung dysplasia is closely related to the changes of EGF EGFR system.