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α_2-巨球蛋白(α_2M)是纤维蛋白溶酶、凝血酶、胰蛋白酶、糜蛋白酶等多种蛋白水解酶的广谱抑制剂,这些蛋白水解酶是在炎性反应中产生的。因此,α_2M在脓毒症这样一些病症治疗中具有重要作用。为了研究α_2M在脓毒症中的状况,作者建立了测定功能性及失活α_M 的方法。血浆中功能性α_2M 的测定是通过α_2M 与固相胰蛋白酶的定量结合,运用放射免疫法(RIA)实现的;失活α_2M 则是通过单克隆抗体-McAbM_1与暴露于α_M 表面的新决定簇的特异性反应,运用RIA 法进行测定的。与健康人相比,48例脓毒症病人血浆中功能性α_M 减少(P<0.0001)。就允许的和最低的功能性α_2M 而言,23例休克病人比其余25例患者的低(分
Alpha-2-macroglobulin (α_2M) is a broad-spectrum inhibitor of various proteolytic enzymes such as plasmin, thrombin, trypsin and chymotrypsin, and these proteolytic enzymes are produced during inflammatory reactions. Therefore, α_2M plays an important role in the treatment of such diseases as sepsis. In order to investigate the condition of α_2M in sepsis, the authors established a method to determine the functionality and inactivation of α_M. The determination of functional α_2M in plasma is achieved by the quantitative combination of α_2M with solid-phase trypsin by radioimmunoassay (RIA). Inactivation of α_2M is mediated by monoclonal antibody -McAbM_1 and new determinants exposed to α_M surface Specific reaction, using RIA method for determination. In 48 sepsis patients, plasma alpha-M was decreased (P <0.0001) compared with healthy controls. For the allowed and lowest functional α_2M, the 23 patients with shock were lower (score than the other 25 patients