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目的采用HPLC法同时测定穿心莲药材及其制剂中6种内酯类成分的含量。方法采用Kromasil RP-C18柱(250mm×4.6 mm,5μm),流动相为乙腈-水,采用二元梯度洗脱,流速1.0 mL.min-1,检测波长226 nm,柱温25℃。结果穿心莲内酯、异穿心莲内酯、新穿心莲内酯、去氧穿心莲内酯、脱水穿心莲内酯和穿心莲宁的平均加样回收率分别为100.5%、100.7%、99.3%、100.8%、100.9%、101.3%;线性范围分别为1.68～67.20μg(r=0.9970)、0.40～16.60μg(r=0.9967)、1.08～43.20μg(r=0.9964)、1.08～43.20μg(r=0.9969)、1.90～76.00μg(r=0.9965)、0.31～12.40μg(r=0.9972)(n=6)。结论所建方法简便可行、重复性良好,可用于穿心莲药材及含穿心莲制剂的质量监控。
Objective To determine the content of six kinds of lactones in Andrographis paniculata by HPLC method. Methods A Kromasil RP-C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-water and eluted with a gradient of 1.0 mL · min-1 at a flow rate of 226 nm. Results The average recoveries of andrographolide, andrographolide, andrographolide, deoxyandrographolide, dehydroandrographolide and andrographolide were 100.5%, 100.7%, 99.3%, 100.8% and 100.9%, respectively And 101.3%, respectively. The linear range was 1.68 ~ 67.20μg (r = 0.9970), 0.40 ~ 16.60μg (r = 0.9967), 1.08 ~ 43.20μg (r = 0.9964) 76.00 μg (r = 0.9965), 0.31-12.40 μg (r = 0.9972) (n = 6). Conclusion The method is simple, feasible and reproducible. It can be used for quality control of Andrographis paniculata and its preparation.