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[目的]评估不同浓度臭氧对类风湿性关节炎大鼠的治疗效果,筛选治疗RA的最佳臭氧浓度。[方法]Wistar大鼠48只,随机分为对照组、模型组、纯氧组、O3-10组、O3-20组、O3-30组、O3-40组、O3-50组,注射胶原蛋白Ⅱ与完全弗氏佐剂建立大鼠类风湿性关节炎模型。第21 d起,根据分组关节内注射纯氧和不同浓度臭氧1 ml,每周1次,共注射3周。观察大鼠关节肿胀情况、双后肢足爪厚度以及滑膜形态学变化。[结果]造模21 d后关节肿胀达到高峰(P<0.01)。治疗结束,O3-40组大鼠已无关节红肿现象,滑膜切片增生不明显,炎性浸润少,O3-40组大鼠足爪厚度与正常组相比无统计学意义(P>0.05)。[结论]关节内注射浓度为40μg/ml臭氧能较好抑制类风湿性关节炎大鼠滑膜炎。
[Objective] To evaluate the therapeutic effects of different concentrations of ozone on rheumatoid arthritis rats and select the best ozone concentration for the treatment of RA. [Methods] 48 Wistar rats were randomly divided into control group, model group, pure oxygen group, O3-10 group, O3-20 group, O3-30 group, O3-40 group, O3-50 group, collagen injection Ⅱ and complete Freund ’s adjuvant to establish rat model of rheumatoid arthritis. The first 21 d, according to intra-articular injection of pure oxygen and different concentrations of ozone 1 ml, once a week for a total of 3 weeks. The swelling of the joints, the thickness of the paws of the hind limbs and the synovial morphological changes were observed. [Result] The joint swelling reached its peak 21 days after modeling (P <0.01). At the end of treatment, O3-40 group had no joint swelling, no obvious synovial hyperplasia and less inflammatory infiltration. There was no significant difference in paw thickness between O3-40 group and normal group (P> 0.05) . [Conclusion] Intra-articular injection of ozone at a concentration of 40 μg / ml can effectively inhibit synovitis in rheumatoid arthritis rats.