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人体免疫缺损病毒的包膜蛋白gp120的V3环区包含一段在人类蛋白质中很少出现的高度保守序列,但这段序列与纤溶酶原被纤溶酶原激活剂酶切位点附近序列有同源性.由于V3环区在人体免疫缺损病毒侵染细胞过程中的重要性,评估了尿激酶对人体免疫缺损病毒侵染能力的影响.通过检测逆转录酶活力,P24抗原的表达和合胞体形成情况发现尿激酶可以抑制人体免疫缺损病毒对多种淋巴瘤和白血病细胞系,如MT4、CEM、H9和外周血单核细胞的侵染能力,并且这种抑制与尿激酶浓度呈剂量依赖关系.那些能够被尿激酶抑制的人体免疫缺损病毒株其V3环区序列必须与纤溶酶原激活区序列同源,实验室常用病毒株包括BRU和RF以及某些野生病毒株.研究结果显示尿激酶在体外实验中可以抑制人体免疫缺损病毒的侵染能力.
The V3 loop region of human immunodeficiency virus envelope protein gp120 contains a highly conserved sequence that rarely occurs in human proteins but this sequence is associated with sequences near the site where plasminogen is cleaved by the plasminogen activator Homology The impact of urokinase on the infectivity of human immunodeficiency virus was assessed as the importance of the V3 loop region in the infection of human immunodeficiency virus by detecting the expression of P24 antigen and syncytia The formation of urokinase was found to inhibit the ability of human immunodeficiency virus to infect a variety of lymphomas and leukemia cell lines such as MT4, CEM, H9 and peripheral blood mononuclear cells in a dose-dependent manner . Those human immunodeficiency virus strains that can be inhibited by urokinase must have their V3 loop sequences homologous to the plasminogen activator region sequences and common lab strains include BRU and RF as well as certain wild virus strains.The results show that urine In vitro kinase inhibition of human immunodeficiency virus infectivity.