论文部分内容阅读
目的探讨中药骨碎补对体外培养的人牙龈成纤维细胞增殖分化的影响。方法将体外培养的人牙龈成纤维细胞与10×103、50×103、100×103、500×103与1000×103μg/L浓度骨碎补共孵育后,测定碱性磷酸酶活性,将最佳浓度骨碎补作用于培养细胞,应用流式细胞仪检测细胞周期的改变。结果在骨碎补各浓度范围内,实验组的OD值均高于对照组(P<0.05),且浓度为100μg/mL时其碱性磷酸酶活性最高,G1期细胞减少,S期和G2M期细胞增多,增殖指数值高于对照组(P<0.05)。结论骨碎补在一定浓度范围内,使体外培养的人牙龈成纤维细胞的碱性磷酸酶活性增强,更多的细胞进入增殖状态。
Objective To investigate the effect of Dried Rhizoma Drynariae on the proliferation and differentiation of human gingival fibroblasts cultured in vitro. Methods Human gingival fibroblasts cultured in vitro were incubated with Rhizoma Drynariae at a concentration of 10 × 103, 50 × 103, 100 × 103, 500 × 103 and 1000 × 103μg / L. The activity of alkaline phosphatase Concentration of dry nodules in cultured cells, the application of flow cytometry to detect cell cycle changes. Results The OD values in the experimental group were significantly higher than those in the control group (P <0.05). The highest alkaline phosphatase activity, the lowest G1 phase cells, the lower S phase and G2M Proliferation of cells, proliferation index value was higher than the control group (P <0.05). Conclusion Drynaria could be supplemented within a certain range of concentration, and alkaline phosphatase activity of human gingival fibroblasts cultured in vitro was enhanced. More cells entered the proliferative state.