论文部分内容阅读
目的利用响应面分析和增强Donnan效应耦合技术对苦参碱的纳滤浓缩工艺进行优化。方法以苦参碱及总生物碱截留率为指标,在明确操作压力、温度、平衡体积等因素影响的基础上,采用Box-Behnken中心组合设计建立数学模型,考察截留相对分子质量、指标成分质量浓度、p H值,得出各因素对截留率的影响程度,设定溶液p H值6~7,以乙醇体积分数调节苦参碱与纳滤膜间的Donnan效应,优选纳滤浓缩参数。结果乙醇体积分数与生物碱截留率呈正相关,优选出纳滤浓缩参数为纳滤膜截留相对分子质量150,p H值6.19,苦参碱质量浓度204.3μg/m L,总生物碱质量浓度603.1μg/m L,乙醇体积分数10%,苦参碱截留率为94.41%,总生物碱截留率97.63%。结论以乙醇溶液调节Donnan效应与响应面法相结合,可用于优选中药纳滤浓缩的参数,为热敏性中药成分的纳滤浓缩提供基础。
Objective To optimize the nanofiltration concentration of matrine by response surface analysis and enhanced Donnan coupling. Methods Taking the retention rate of matrine and total alkaloids as an index, a mathematical model was set up based on the combination of Box-Behnken center design based on the effects of operating pressure, temperature and equilibrium volume. The relative molecular weight and the quality of indicator components Concentration, and p H values. The influence of each factor on the rejection rate was obtained. The p H value of the solution was set to 6-7, and the Donnan effect between matrine and the nanofiltration membrane was adjusted by ethanol volume fraction. Results The volume fraction of ethanol was positively correlated with the retention of alkaloids. The optimal parameters for nanofiltration were nanofiltration membrane with molecular weight cut-off of 150, p H value of 6.19, matrine concentration of 204.3 μg / m L, total alkaloid concentration of 603.1 μg / m L, ethanol volume fraction of 10%, matrine retention rate of 94.41%, total alkaloid retention rate of 97.63%. Conclusion The combination of Donnan effect and response surface method with ethanol solution can be used to optimize the concentration parameters of nanofiltration in traditional Chinese medicine and provide the basis for the nanofiltration concentration of thermosensitive traditional Chinese medicine components.